Saccharomyces boulardii activates expression of peroxisome proliferator-activated receptor-gamma in HT-29 cells

Sang Kil Lee, Hyo Jong Kim, Sung Gil Chi, Jae Young Jang, Ki Deok Nam, Nam Hoon Kim, Kwang Ro Joo, Seok Ho Dong, Byung Ho Kim, Young Woon Chang, Joung Il Lee, Rin Chang

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Abstract

BACKGROUND/AIMS: Saccharomyces boulardii (S. boulardii) has been reported to be beneficial in the treatment of inflammatory bowel disease, however, little is known about its mechanism of action. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is recently found to regulate inflammation in intestinal epithelial cells. We hypothesized that the anti-inflammatory effects of S. boulardii are mediated, in part, through PPAR-gamma. To test this hypothesis, we examined the ability of S. boulardii to modulate the expression of PPAR-gamma in human colon cells. METHODS: Effects of S. boulardii on survival and proliferation of HT-29 human colon cells were assessed by MTT and [3H]thymidine incorporation assays. PPAR-gamma expression was assessed by Western blot and RT-PCR. Induction of interleukin-8 (IL-8) expression by tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), or lipopolysaccharide (LPS) was assessed by RT-PCR. RESULTS: S. boulardii did not affect viability and proliferation of HT-29 cells. S. boulardii up-regulated PPAR-gamma expression at both mRNA and protein levels. Pretreatment of HT-29 cells with S. boulardii blocked PPAR-gamma down-regulation by TNF-alpha, IL-1beta, or LPS, whereas it ameliorated IL-8 response to these proinflammatory factors. CONCLUSIONS: S. boulardii stimulates PPAR-gamma expression and reduces response of human colon cells to proinflammatory cytokines.

Original languageEnglish
Pages (from-to)328-334
Number of pages7
JournalThe Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi
Volume45
Issue number5
Publication statusPublished - 2005 May

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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    Lee, S. K., Kim, H. J., Chi, S. G., Jang, J. Y., Nam, K. D., Kim, N. H., Joo, K. R., Dong, S. H., Kim, B. H., Chang, Y. W., Lee, J. I., & Chang, R. (2005). Saccharomyces boulardii activates expression of peroxisome proliferator-activated receptor-gamma in HT-29 cells. The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi, 45(5), 328-334.