Sargaquinoic acid and sargachromenol, extracts of Sargassum sagamianum, induce apoptosis in HaCaT cells and mice skin

Its potentiation of UVB-induced apoptosis

Seulgi Hur, Hyangkyu Lee, Younghwa Kim, Bong Ho Lee, Jongheon Shin, Tae Yoon Kim

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The plastoquinones sargaquinoic acid and sargachromenol are major components of brown alga Sargassum sagamianum and are known to be involved in neurite growth and survival. In this study, we describe their novel pro-apoptotic activities in vitro and in vivo. In vitro, treatment with sargaquinoic acid or sargachromenol promoted cell death and activation of caspase-3, caspase-8, caspase-9 and poly (ADP-ribose) polymerase (PARP) in a concentration-dependent manner. Sargaquinoic acid- or sargachromenol-induced apoptosis was enhanced by co-treatment with UVB irradiation. It showed much higher levels of cleaved caspase-3, caspase-8, caspase-9, and PARP than with sargaquinoic acid and sargachromenol alone, while it had no effect on Bcl-2 and Bax expression level. Examination by terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) and immunohistochemistry showed that topical application of sargaquinoic acid (1 mg/ml) before UVB irradiation (2.5 kJ/m 2 ) of hairless mice also enhanced apoptosis including activation of caspase-3. Since a combination of phototherapy using UVB with topical reagents has been clinically applied to treat hyperproliferative skin disease, these results suggest that sargaquinoic acid and sargachromenol could be effective therapeutic agents.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalEuropean Journal of Pharmacology
Volume582
Issue number1-3
DOIs
Publication statusPublished - 2008 Mar 17

Fingerprint

Sargassum
Apoptosis
Skin
Caspase 3
Poly(ADP-ribose) Polymerases
Caspase 9
Caspase 8
Plastoquinone
Phaeophyta
Hairless Mouse
Phototherapy
DNA Nucleotidylexotransferase
In Situ Nick-End Labeling
Neurites
Skin Diseases
sargachromenol
sargaquinoic acid
Cell Death
Therapeutics
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

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title = "Sargaquinoic acid and sargachromenol, extracts of Sargassum sagamianum, induce apoptosis in HaCaT cells and mice skin: Its potentiation of UVB-induced apoptosis",
abstract = "The plastoquinones sargaquinoic acid and sargachromenol are major components of brown alga Sargassum sagamianum and are known to be involved in neurite growth and survival. In this study, we describe their novel pro-apoptotic activities in vitro and in vivo. In vitro, treatment with sargaquinoic acid or sargachromenol promoted cell death and activation of caspase-3, caspase-8, caspase-9 and poly (ADP-ribose) polymerase (PARP) in a concentration-dependent manner. Sargaquinoic acid- or sargachromenol-induced apoptosis was enhanced by co-treatment with UVB irradiation. It showed much higher levels of cleaved caspase-3, caspase-8, caspase-9, and PARP than with sargaquinoic acid and sargachromenol alone, while it had no effect on Bcl-2 and Bax expression level. Examination by terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) and immunohistochemistry showed that topical application of sargaquinoic acid (1 mg/ml) before UVB irradiation (2.5 kJ/m 2 ) of hairless mice also enhanced apoptosis including activation of caspase-3. Since a combination of phototherapy using UVB with topical reagents has been clinically applied to treat hyperproliferative skin disease, these results suggest that sargaquinoic acid and sargachromenol could be effective therapeutic agents.",
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Sargaquinoic acid and sargachromenol, extracts of Sargassum sagamianum, induce apoptosis in HaCaT cells and mice skin : Its potentiation of UVB-induced apoptosis. / Hur, Seulgi; Lee, Hyangkyu; Kim, Younghwa; Lee, Bong Ho; Shin, Jongheon; Kim, Tae Yoon.

In: European Journal of Pharmacology, Vol. 582, No. 1-3, 17.03.2008, p. 1-11.

Research output: Contribution to journalArticle

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