Sarpogrelate hydrochloride ameliorates diabetic nephropathy associated with inhibition of macrophage activity and inflammatory reaction in db/db mice

Eun Soo Lee, Mi Young Lee, Mi Hye Kwon, Hong Min Kim, Jeong Suk Kang, You Mi Kim, Eun Young Lee, Choon Hee Chung

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this study was to evaluate the effects of sarpogrelate hydrochloride (SH), a selective serotonin 2A receptor antagonist, on diabetic nephropathy in a type 2 diabetes mouse model. We treated db/m and db/db mice with SH (30 mg/kg/day) for 12 weeks. Rat renal proximal tubule cells (NRK-52E) and mouse macrophages (Raw 264.7) were stimulated by high glucose (30 mM glucose) or LPS (100 ng/ml) with or without SH (20 μM). We found that SH treatment increased serum adiponectin level and decreased urinary albumin, macrophage infiltration to glomeruli, and renal inflammatory and fibrosis signals, which were highly expressed in diabetic mice. Proximal tubule cells treated with high glucose (30 mM) also showed increased inflammatory and fibrosis signals. However, SH (20 μM) treatment reduced these changes. Moreover, SH treatment inhibited LPS-stimulated macrophage migration and activation. These findings suggest that SH ameliorates diabetic nephropathy not only by suppressing macrophage infiltration, but also by anti-inflammatory and antifibrotic effects.

Original languageEnglish
Article numbere0179221
JournalPloS one
Volume12
Issue number6
DOIs
Publication statusPublished - 2017 Jun

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diabetic nephropathy
Macrophages
Diabetic Nephropathies
macrophages
proximal tubules
mice
fibrosis
glucose
kidneys
adiponectin
Infiltration
Glucose
noninsulin-dependent diabetes mellitus
serotonin
Fibrosis
albumins
antagonists
animal models
Receptor, Serotonin, 5-HT2A
Proximal Kidney Tubule

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Lee, Eun Soo ; Lee, Mi Young ; Kwon, Mi Hye ; Kim, Hong Min ; Kang, Jeong Suk ; Kim, You Mi ; Lee, Eun Young ; Chung, Choon Hee. / Sarpogrelate hydrochloride ameliorates diabetic nephropathy associated with inhibition of macrophage activity and inflammatory reaction in db/db mice. In: PloS one. 2017 ; Vol. 12, No. 6.
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Sarpogrelate hydrochloride ameliorates diabetic nephropathy associated with inhibition of macrophage activity and inflammatory reaction in db/db mice. / Lee, Eun Soo; Lee, Mi Young; Kwon, Mi Hye; Kim, Hong Min; Kang, Jeong Suk; Kim, You Mi; Lee, Eun Young; Chung, Choon Hee.

In: PloS one, Vol. 12, No. 6, e0179221, 06.2017.

Research output: Contribution to journalArticle

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