Sauchinone attenuates oxidative stress-induced skeletal muscle myoblast damage through the down-regulation of ceramide

Min Ho Jung, Min Cheol Song, Kiho Bae, Han Sung Kim, Seung Hyun Kim, Sang Hyun Sung, Sang Kyu Ye, Kwang Ho Lee, Yeo Pyo Yun, Tack Joong Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We investigated the effects of sauchinone, isolated from the root of Saururus chinensis, on muscle disorders and the underlying mechanism of oxidative stress-induced C 2C 12 skeletal muscle myoblast damage. To assess the protective effects of sauchinone on oxidative stress-induced C 2C 12 skeletal muscle myoblasts, we measured the viability of the cells, showing that sauchinone pre-treatment significantly reduced the decreased cell viability after H 2O 2 treatment. We also investigated the mechanism of this protective effect of sauchinone. In Western blot analysis, the heat shock protein (HSP)-70 level increased significantly in the sauchinone-pretreated myoblasts. We used high performance liquid chromatography (HPLC) to examine the level of endogenous ceramide after pre-treatment with sauchinone followed by exposure to H 2O 2. While hydrogen peroxide increased the ceramide content to approximately 166.60±38.93% of the control level, pre-treatment with sauchinone inhibited this increase, maintaining the ceramide content at the control level. We demonstrated that sauchinone regulates intracellular HSP70 expression as well as ceramide levels to protect against oxidative stress-induced C 2C 12 muscle myoblast damage. We suggest the potential benefits of herbal medicines in the treatment of oxidative stress-related muscle disorders.

Original languageEnglish
Pages (from-to)575-579
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume34
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

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Skeletal Myoblasts
Ceramides
Skeletal Muscle
Oxidative Stress
Down-Regulation
Myoblasts
Muscular Diseases
Saururaceae
Cell Survival
HSP70 Heat-Shock Proteins
sauchinone
Herbal Medicine
Hydrogen Peroxide
Western Blotting
High Pressure Liquid Chromatography
Muscles

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Jung, Min Ho ; Song, Min Cheol ; Bae, Kiho ; Kim, Han Sung ; Kim, Seung Hyun ; Sung, Sang Hyun ; Ye, Sang Kyu ; Lee, Kwang Ho ; Yun, Yeo Pyo ; Kim, Tack Joong. / Sauchinone attenuates oxidative stress-induced skeletal muscle myoblast damage through the down-regulation of ceramide. In: Biological and Pharmaceutical Bulletin. 2011 ; Vol. 34, No. 4. pp. 575-579.
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Sauchinone attenuates oxidative stress-induced skeletal muscle myoblast damage through the down-regulation of ceramide. / Jung, Min Ho; Song, Min Cheol; Bae, Kiho; Kim, Han Sung; Kim, Seung Hyun; Sung, Sang Hyun; Ye, Sang Kyu; Lee, Kwang Ho; Yun, Yeo Pyo; Kim, Tack Joong.

In: Biological and Pharmaceutical Bulletin, Vol. 34, No. 4, 01.04.2011, p. 575-579.

Research output: Contribution to journalArticle

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AU - Jung, Min Ho

AU - Song, Min Cheol

AU - Bae, Kiho

AU - Kim, Han Sung

AU - Kim, Seung Hyun

AU - Sung, Sang Hyun

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AU - Kim, Tack Joong

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