Scalable long-term extraction of photosynthetic electrons by simple sandwiching of nanoelectrode array with densely-packed algal cell film

Yong Jae Kim, Jae Hyoung Yun, Seon Il Kim, Hyeonaug Hong, Jun Hee Park, Jae Chul Pyun, WonHyoung Ryu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Direct extraction of photosynthetic electrons from the whole photosynthetic cells such as plant cells or algal cells can be highly efficient and sustainable compared to other approaches based on isolated photosynthetic apparatus such as photosystems I, II, and thylakoid membranes. However, insertion of nanoelectrodes (NEs) into individual cells are time-consuming and unsuitable for scale-up processes. We propose simple and efficient insertion of massively-populated NEs into cell films in which algal cells are densely packed in a monolayer. After stacking the cell film over an NE array, gentle pressing of the stack allows a large number of NEs to be inserted into the cells in the cell film. The NE array was fabricated by metal-assisted chemical etching (MAC-etching) followed by additional steps of wet oxidation and oxide etching. The cell film was prepared by mixing highly concentrated algal cells with alginate hydrogel. Photosynthetic currents of up to 106 nA/cm 2 was achieved without aid of mediators, and the photosynthetic function was maintained for 6 days after NE array insertion into algal cells.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalBiosensors and Bioelectronics
Volume117
DOIs
Publication statusPublished - 2018 Oct 15

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Motion Pictures
Electrons
Etching
Photosystem I Protein Complex
Hydrogel
Alginate
Hydrogels
Oxides
Monolayers
Metals
Membranes
Oxidation
Thylakoids
Photosystem II Protein Complex
Plant Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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title = "Scalable long-term extraction of photosynthetic electrons by simple sandwiching of nanoelectrode array with densely-packed algal cell film",
abstract = "Direct extraction of photosynthetic electrons from the whole photosynthetic cells such as plant cells or algal cells can be highly efficient and sustainable compared to other approaches based on isolated photosynthetic apparatus such as photosystems I, II, and thylakoid membranes. However, insertion of nanoelectrodes (NEs) into individual cells are time-consuming and unsuitable for scale-up processes. We propose simple and efficient insertion of massively-populated NEs into cell films in which algal cells are densely packed in a monolayer. After stacking the cell film over an NE array, gentle pressing of the stack allows a large number of NEs to be inserted into the cells in the cell film. The NE array was fabricated by metal-assisted chemical etching (MAC-etching) followed by additional steps of wet oxidation and oxide etching. The cell film was prepared by mixing highly concentrated algal cells with alginate hydrogel. Photosynthetic currents of up to 106 nA/cm 2 was achieved without aid of mediators, and the photosynthetic function was maintained for 6 days after NE array insertion into algal cells.",
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Scalable long-term extraction of photosynthetic electrons by simple sandwiching of nanoelectrode array with densely-packed algal cell film. / Kim, Yong Jae; Yun, Jae Hyoung; Kim, Seon Il; Hong, Hyeonaug; Park, Jun Hee; Pyun, Jae Chul; Ryu, WonHyoung.

In: Biosensors and Bioelectronics, Vol. 117, 15.10.2018, p. 15-22.

Research output: Contribution to journalArticle

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