Scopolin ameliorates high-fat diet induced hepatic steatosis in mice

Potential involvement of SIRT1-mediated signaling cascades in the liver

Ahyoung Yoo, Vikram P. Narayan, Eun Young Hong, Wan Kyunn Whang, Taesun Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The present study aimed to investigate whether scopolin exhibits beneficial effects on high-fat diet (HFD)-induced hepatic steatosis in mice. The involvement of sirtuin 1 (SIRT1) as a molecular target for scopolin was also explored. Scopolin decreased the Km of SIRT1 for p53 and nicotinamide adenine dinucleotide without altering Vmax in a cell-free system. Scopolin alleviated oleic acid-induced lipid accumulation and downregulation of SIRT1 activity in HepG2 cells, and these beneficial effects of scopolin were abolished in the presence of SIRT1 inhibitor. Mice administered 0.02% scopolin for 8 weeks exhibited improved phenotypes of HFD-induced hepatic steatosis along with increased hepatic SIRT1 activity and protein expression. Scopolin resulted in increased deacetylation of sterol regulatory element-binding protein 1c with subsequent downregulation of lipogenic genes, and enhanced deacetylation of protein peroxisome proliferator-activated receptor-3 coactivator 1α with upregulation of fatty acid oxidation genes in livers. Scopolin also enhanced deacetylation of nuclear factor-kappa enhancer binding protein and liver kinase B1 (LKB1), facilitating LKB1/AMP-activated protein kinase signaling cascades. Scopolin attenuated hepatic steatosis through activation of SIRT1-mediated signaling cascades, a potent regulator of lipid homeostasis. Increased hepatic SIRT1 activity and protein expression appeared to be associated with these beneficial effects of scopolin.

Original languageEnglish
Article number2251
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Sirtuin 1
High Fat Diet
Liver
Down-Regulation
scopolin
Sterol Regulatory Element Binding Protein 1
Lipids
Peroxisome Proliferator-Activated Receptors
Proteins
Cell-Free System
Hep G2 Cells
Oleic Acid
NAD
Protein Kinases
Genes
Carrier Proteins
Homeostasis
Up-Regulation
Fatty Acids

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Scopolin ameliorates high-fat diet induced hepatic steatosis in mice: Potential involvement of SIRT1-mediated signaling cascades in the liver",
abstract = "The present study aimed to investigate whether scopolin exhibits beneficial effects on high-fat diet (HFD)-induced hepatic steatosis in mice. The involvement of sirtuin 1 (SIRT1) as a molecular target for scopolin was also explored. Scopolin decreased the Km of SIRT1 for p53 and nicotinamide adenine dinucleotide without altering Vmax in a cell-free system. Scopolin alleviated oleic acid-induced lipid accumulation and downregulation of SIRT1 activity in HepG2 cells, and these beneficial effects of scopolin were abolished in the presence of SIRT1 inhibitor. Mice administered 0.02{\%} scopolin for 8 weeks exhibited improved phenotypes of HFD-induced hepatic steatosis along with increased hepatic SIRT1 activity and protein expression. Scopolin resulted in increased deacetylation of sterol regulatory element-binding protein 1c with subsequent downregulation of lipogenic genes, and enhanced deacetylation of protein peroxisome proliferator-activated receptor-3 coactivator 1α with upregulation of fatty acid oxidation genes in livers. Scopolin also enhanced deacetylation of nuclear factor-kappa enhancer binding protein and liver kinase B1 (LKB1), facilitating LKB1/AMP-activated protein kinase signaling cascades. Scopolin attenuated hepatic steatosis through activation of SIRT1-mediated signaling cascades, a potent regulator of lipid homeostasis. Increased hepatic SIRT1 activity and protein expression appeared to be associated with these beneficial effects of scopolin.",
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Scopolin ameliorates high-fat diet induced hepatic steatosis in mice : Potential involvement of SIRT1-mediated signaling cascades in the liver. / Yoo, Ahyoung; Narayan, Vikram P.; Hong, Eun Young; Whang, Wan Kyunn; Park, Taesun.

In: Scientific Reports, Vol. 7, No. 1, 2251, 01.12.2017.

Research output: Contribution to journalArticle

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T2 - Potential involvement of SIRT1-mediated signaling cascades in the liver

AU - Yoo, Ahyoung

AU - Narayan, Vikram P.

AU - Hong, Eun Young

AU - Whang, Wan Kyunn

AU - Park, Taesun

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