Screening of Indonesian medicinal plants for inhibitor activity on nitric oxide production of RAW264.7 cells and antioxidant activity

Eun Mi Choi, Jae Kwan Hwang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Traditional Indonesian medicinal plants were screened for their inhibitory effects on the nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and for the antioxidant activity through the evaluation of free radical scavenging effect and reducing power. The results of screening indicated that 50 methanolic extracts inhibited (>50%) lipopolysaccharides (LPS)-induced NO release from RAW264.7 cells at 50 μg/ml, with 18 having greater than 100% inhibition. At 200 μg/ml, 61 methanol extracts exhibited inhibitory activity (>50%), with 45 showing greater than 100% inhibition. In addition, the free radical scavenging effects of 6 methanolic extracts were found to be more than 50% for extract concentration of 0.5 μg/ml. The results indicate that the extracts contain active compounds that inhibit NO release and scavenge free radical.

Original languageEnglish
Pages (from-to)194-203
Number of pages10
JournalFitoterapia
Volume76
Issue number2
DOIs
Publication statusPublished - 2005 Mar

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Medicinal Plants
Free Radicals
Nitric Oxide
Antioxidants
Lipopolysaccharides
Methanol
Macrophages

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

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abstract = "Traditional Indonesian medicinal plants were screened for their inhibitory effects on the nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and for the antioxidant activity through the evaluation of free radical scavenging effect and reducing power. The results of screening indicated that 50 methanolic extracts inhibited (>50{\%}) lipopolysaccharides (LPS)-induced NO release from RAW264.7 cells at 50 μg/ml, with 18 having greater than 100{\%} inhibition. At 200 μg/ml, 61 methanol extracts exhibited inhibitory activity (>50{\%}), with 45 showing greater than 100{\%} inhibition. In addition, the free radical scavenging effects of 6 methanolic extracts were found to be more than 50{\%} for extract concentration of 0.5 μg/ml. The results indicate that the extracts contain active compounds that inhibit NO release and scavenge free radical.",
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Screening of Indonesian medicinal plants for inhibitor activity on nitric oxide production of RAW264.7 cells and antioxidant activity. / Choi, Eun Mi; Hwang, Jae Kwan.

In: Fitoterapia, Vol. 76, No. 2, 03.2005, p. 194-203.

Research output: Contribution to journalArticle

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