Abstract
This paper describes a novel method for generating 3D freestanding lipid bilayers (3D FLBs) on a transparent microwell arrays and their sealing. The 3D FLBs have better stability and larger surface area due to their cell-like structure. To improve sealing characteristics, the applied AC frequency was controlled during the electroformation. The 3D FLBs were observed through SU-8 microwell using confocal microscopy, resulting in perfect sealing at more than 1 kHz, and also discussed details in sealing of fixed 3D lipid structure for the first time. Unilamellarity and biofunctionality of the 3D FLBs were verified by transport protein assay.
| Original language | English |
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| Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1401-1403 |
| Number of pages | 3 |
| ISBN (Electronic) | 9781510897571 |
| Publication status | Published - 2018 |
| Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 2018 Nov 11 → 2018 Nov 15 |
Publication series
| Name | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Volume | 3 |
Conference
| Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
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| Country/Territory | Taiwan, Province of China |
| City | Kaohsiung |
| Period | 18/11/11 → 18/11/15 |
Bibliographical note
Funding Information:This research was supported by KIST (Korea Institute of Science and Technology) Institutional Program (2E27910). We are also indebted to Dr. Young Jun Kim and Dr. Kuiwon Choi, Director, Korea Institute of Science and Technology Europe, Germany for the alpha-hemolysin experiments.
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Bioengineering
- Chemical Engineering (miscellaneous)
- Control and Systems Engineering