Seasonal variation in Daucus carota leaf-surface and leaf-tissue chemical profiles

Janie S. Brooks, Paul Feeny

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The purpose of this work was to document seasonal changes in leaf-surface and whole-leaf chemistry of Daucus carota cohorts that differed in life-cycle phenology (winter annual, annual, or biennial), with particular focus on compounds that serve as contact oviposition stimulants for Papilio polyxenes, the black swallowtail butterfly. Cohorts of carrot plants exhibiting different life-cycle phenologies were established, and plants from each cohort were sampled periodically over two growing seasons. Extracts of leaf surfaces and leaf tissues were analyzed using HPLC and were compared quantitatively for the content of chlorogenic acid (CA), luteolin-7-O-(6″-O-malonyl)-β-D- glucopyranoside (L7MG), and luteolin-7-glucoside (L7G). The pattern of seasonal variation in L7G and L7MG concentrations in leaf tissues of each cohort followed the predictions of the tissue value hypothesis: flavonoid concentrations are highest in the tissues of new alternate leaves of the reproductive shoot or in new spring foliage. Chlorogenic acid concentrations in leaf tissues increased throughout the growing season. Patterns of leaf-surface seasonal variation did not correlate with patterns in leaf-tissue chemistry for any carrot cohort. Rather, leaf-surface amounts of CA, L7G, and L7MG were highest at the point in the lifecycle when plants were differentiating or bolting.

Original languageEnglish
Pages (from-to)769-782
Number of pages14
JournalBiochemical Systematics and Ecology
Volume32
Issue number9
DOIs
Publication statusPublished - 2004 Sep 1

Fingerprint

Daucus carota
seasonal variation
Tissue
Chlorogenic Acid
luteolin
leaves
Life Cycle Stages
Papilio polyxenes
chlorogenic acid
Life cycle
glucosides
Luteolin
Butterflies
Oviposition
carrots
acid
life cycle (organisms)
Flavonoids
chemistry
growing season

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

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Seasonal variation in Daucus carota leaf-surface and leaf-tissue chemical profiles. / Brooks, Janie S.; Feeny, Paul.

In: Biochemical Systematics and Ecology, Vol. 32, No. 9, 01.09.2004, p. 769-782.

Research output: Contribution to journalArticle

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