Secretory production of recombinant proteins in Escherichia coli

Sung Ho Yoon, Seong Keun Kim, Jihyun F. Kim

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)

Abstract

Extracellular production of heterologous proteins using the Escherichia coli cell factory offers several advantages over intracellular production and mammalian culture. Properly folded proteins can be rapidly accumulated in the culture media, and downstream processes for isolation and purification can be much simplified. Efforts to enhance the secretory production of target proteins can be largely classified as selection and modification of the signal peptide, coexpression of proteins to assist translocation and folding, improvement of periplasmic release, and protection of target proteins from degradation and contamination. Here, we review recent patents on the secretory production of recombinant proteins in E. coli.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalRecent Patents on Biotechnology
Volume4
Issue number1
DOIs
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Secretory production of recombinant proteins in Escherichia coli'. Together they form a unique fingerprint.

Cite this