Selective Energy Transfer Between Quantum Dots and Gold Nanoparticles for Detection of Multiple Mutations in Epidermal Growth Factor Receptor

Hosub Lee, Ahreum Kim, Taegyeong Kang, Sang Woo Joo, So Yeong Lee, Kyong Ah Yoon, Kangtaek Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Selective energy transfer between quantum dots and gold nanoparticles was used to simultaneously detect mutations in the epidermal growth factor receptor (EGFR) gene. We functionalized the surface of gold nanoparticles and green and red-emitting quantum dots using four different probe DNAs that were designed to be a perfect complementary to an in-frame deletion mutation in exon 19 or L858 R point mutation in exon 21 of EGFR. We found that the presence of the deletion mutation in exon 19 in target oligonucleotides caused fluorescence quenching at 525 nm due to energy transfer from green-emitting quantum dots to gold nanoparticles, whereas point mutation in exon 21 resulted in quenching at 620 nm due to energy transfer from red-emitting quantum dots to gold nanoparticles. This method could successfully be used to simultaneously detect the presence of two types of mutations in EGFR. We also defined a parameter (i.e., the extent of quenching) to quantify fluorescence quenching phenomenon. By varying the fraction of mutant type DNA in target oligonucleotides, we showed that detection sensitivity based on the extent of quenching was about 5%, which is lower than the conventional direct sequencing method.

Original languageEnglish
Pages (from-to)2707-2716
Number of pages10
JournalAnalytical Letters
Volume45
Issue number18
DOIs
Publication statusPublished - 2012 Nov 1

Fingerprint

Quantum Dots
Energy Transfer
Epidermal Growth Factor Receptor
Gold
Nanoparticles
Energy transfer
Semiconductor quantum dots
Exons
Quenching
Mutation
Sequence Deletion
Point Mutation
Oligonucleotides
Fluorescence
erbB-1 Genes
DNA
DNA Probes
Genes
Epidermal Growth Factor

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

Cite this

Lee, Hosub ; Kim, Ahreum ; Kang, Taegyeong ; Joo, Sang Woo ; Lee, So Yeong ; Yoon, Kyong Ah ; Lee, Kangtaek. / Selective Energy Transfer Between Quantum Dots and Gold Nanoparticles for Detection of Multiple Mutations in Epidermal Growth Factor Receptor. In: Analytical Letters. 2012 ; Vol. 45, No. 18. pp. 2707-2716.
@article{035af9c785344ff590fc2ae53a118228,
title = "Selective Energy Transfer Between Quantum Dots and Gold Nanoparticles for Detection of Multiple Mutations in Epidermal Growth Factor Receptor",
abstract = "Selective energy transfer between quantum dots and gold nanoparticles was used to simultaneously detect mutations in the epidermal growth factor receptor (EGFR) gene. We functionalized the surface of gold nanoparticles and green and red-emitting quantum dots using four different probe DNAs that were designed to be a perfect complementary to an in-frame deletion mutation in exon 19 or L858 R point mutation in exon 21 of EGFR. We found that the presence of the deletion mutation in exon 19 in target oligonucleotides caused fluorescence quenching at 525 nm due to energy transfer from green-emitting quantum dots to gold nanoparticles, whereas point mutation in exon 21 resulted in quenching at 620 nm due to energy transfer from red-emitting quantum dots to gold nanoparticles. This method could successfully be used to simultaneously detect the presence of two types of mutations in EGFR. We also defined a parameter (i.e., the extent of quenching) to quantify fluorescence quenching phenomenon. By varying the fraction of mutant type DNA in target oligonucleotides, we showed that detection sensitivity based on the extent of quenching was about 5{\%}, which is lower than the conventional direct sequencing method.",
author = "Hosub Lee and Ahreum Kim and Taegyeong Kang and Joo, {Sang Woo} and Lee, {So Yeong} and Yoon, {Kyong Ah} and Kangtaek Lee",
year = "2012",
month = "11",
day = "1",
doi = "10.1080/00032719.2012.702177",
language = "English",
volume = "45",
pages = "2707--2716",
journal = "Analytical Letters",
issn = "0003-2719",
publisher = "Taylor and Francis Ltd.",
number = "18",

}

Selective Energy Transfer Between Quantum Dots and Gold Nanoparticles for Detection of Multiple Mutations in Epidermal Growth Factor Receptor. / Lee, Hosub; Kim, Ahreum; Kang, Taegyeong; Joo, Sang Woo; Lee, So Yeong; Yoon, Kyong Ah; Lee, Kangtaek.

In: Analytical Letters, Vol. 45, No. 18, 01.11.2012, p. 2707-2716.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selective Energy Transfer Between Quantum Dots and Gold Nanoparticles for Detection of Multiple Mutations in Epidermal Growth Factor Receptor

AU - Lee, Hosub

AU - Kim, Ahreum

AU - Kang, Taegyeong

AU - Joo, Sang Woo

AU - Lee, So Yeong

AU - Yoon, Kyong Ah

AU - Lee, Kangtaek

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Selective energy transfer between quantum dots and gold nanoparticles was used to simultaneously detect mutations in the epidermal growth factor receptor (EGFR) gene. We functionalized the surface of gold nanoparticles and green and red-emitting quantum dots using four different probe DNAs that were designed to be a perfect complementary to an in-frame deletion mutation in exon 19 or L858 R point mutation in exon 21 of EGFR. We found that the presence of the deletion mutation in exon 19 in target oligonucleotides caused fluorescence quenching at 525 nm due to energy transfer from green-emitting quantum dots to gold nanoparticles, whereas point mutation in exon 21 resulted in quenching at 620 nm due to energy transfer from red-emitting quantum dots to gold nanoparticles. This method could successfully be used to simultaneously detect the presence of two types of mutations in EGFR. We also defined a parameter (i.e., the extent of quenching) to quantify fluorescence quenching phenomenon. By varying the fraction of mutant type DNA in target oligonucleotides, we showed that detection sensitivity based on the extent of quenching was about 5%, which is lower than the conventional direct sequencing method.

AB - Selective energy transfer between quantum dots and gold nanoparticles was used to simultaneously detect mutations in the epidermal growth factor receptor (EGFR) gene. We functionalized the surface of gold nanoparticles and green and red-emitting quantum dots using four different probe DNAs that were designed to be a perfect complementary to an in-frame deletion mutation in exon 19 or L858 R point mutation in exon 21 of EGFR. We found that the presence of the deletion mutation in exon 19 in target oligonucleotides caused fluorescence quenching at 525 nm due to energy transfer from green-emitting quantum dots to gold nanoparticles, whereas point mutation in exon 21 resulted in quenching at 620 nm due to energy transfer from red-emitting quantum dots to gold nanoparticles. This method could successfully be used to simultaneously detect the presence of two types of mutations in EGFR. We also defined a parameter (i.e., the extent of quenching) to quantify fluorescence quenching phenomenon. By varying the fraction of mutant type DNA in target oligonucleotides, we showed that detection sensitivity based on the extent of quenching was about 5%, which is lower than the conventional direct sequencing method.

UR - http://www.scopus.com/inward/record.url?scp=84870433044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870433044&partnerID=8YFLogxK

U2 - 10.1080/00032719.2012.702177

DO - 10.1080/00032719.2012.702177

M3 - Article

AN - SCOPUS:84870433044

VL - 45

SP - 2707

EP - 2716

JO - Analytical Letters

JF - Analytical Letters

SN - 0003-2719

IS - 18

ER -