Selective killing effects of cold atmospheric pressure plasma with no induced dysfunction of epidermal growth factor receptor in oral squamous cell carcinoma

Jung Hwan Lee, Ji Yeon Om, Yong Hee Kim, Kwang Mahn Kim, Eun Ha Choi, Kyoung Nam Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.

Original languageEnglish
Article numbere0150279
JournalPloS one
Volume11
Issue number2
DOIs
Publication statusPublished - 2016 Feb

Fingerprint

Atmospheric Pressure
atmospheric pressure
squamous cell carcinoma
Epidermal Growth Factor Receptor
Atmospheric pressure
Squamous Cell Carcinoma
mouth
Plasmas
Cell culture
nitric oxide
Culture Media
Nitric Oxide
cell culture
Cell Culture Techniques
culture media
Degradation
Oncology
degradation
Fibroblasts
Plasma Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{69f9077a956847e7a6ed37ba6d5f48de,
title = "Selective killing effects of cold atmospheric pressure plasma with no induced dysfunction of epidermal growth factor receptor in oral squamous cell carcinoma",
abstract = "The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.",
author = "Lee, {Jung Hwan} and Om, {Ji Yeon} and Kim, {Yong Hee} and Kim, {Kwang Mahn} and Choi, {Eun Ha} and Kim, {Kyoung Nam}",
year = "2016",
month = "2",
doi = "10.1371/journal.pone.0150279",
language = "English",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

Selective killing effects of cold atmospheric pressure plasma with no induced dysfunction of epidermal growth factor receptor in oral squamous cell carcinoma. / Lee, Jung Hwan; Om, Ji Yeon; Kim, Yong Hee; Kim, Kwang Mahn; Choi, Eun Ha; Kim, Kyoung Nam.

In: PloS one, Vol. 11, No. 2, e0150279, 02.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selective killing effects of cold atmospheric pressure plasma with no induced dysfunction of epidermal growth factor receptor in oral squamous cell carcinoma

AU - Lee, Jung Hwan

AU - Om, Ji Yeon

AU - Kim, Yong Hee

AU - Kim, Kwang Mahn

AU - Choi, Eun Ha

AU - Kim, Kyoung Nam

PY - 2016/2

Y1 - 2016/2

N2 - The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.

AB - The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.

UR - http://www.scopus.com/inward/record.url?scp=84960412002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960412002&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0150279

DO - 10.1371/journal.pone.0150279

M3 - Article

C2 - 26919318

AN - SCOPUS:84960412002

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e0150279

ER -