Selective killing of melanoma cells with non-thermal atmospheric pressure plasma and p-FAK antibody conjugated gold nanoparticles

Byul Bo Ra Choi, Jeong Hae Choi, Jin Woo Hong, Ki Won Song, Hae June Lee, Uk Kyu Kim, Gyoo Cheon Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Melanomas are fast growing high-mortality tumors, and specific treatments for melanomas are needed. Melanoma cells overexpress focal adhesion kinase (FAK) compared to normal keratinocytes, and we sought to exploit this difference to create a selectively lethal therapy. We combined gold nanoparticles (GNP) with antibodies targeting phosphorylated FAK (p-FAK). These conjugates (p-FAK-GNP) entered G361 melanoma cells and bound p-FAK. Treatment with p-FAK-GNP decreased the viability of G361 cells in a time dependent manner by inducing apoptosis. To maximize the preferential killing of G361 cells, non-thermal atmospheric pressure plasma was used to stimulate the GNP within p-FAK-GNP. Combined treatment with plasma and p-FAK-GNP showed much higher lethality against G361 cells than HaCaT keratinocyte cells. The p-FAK-GNP induced apoptosis over 48 hours in G361 cells, whereas plasma and p-FAK-GNP killed G361 cells immediately. This study demonstrates that combining plasma with p-FAK-GNP results in selective lethality against human melanoma cells.

Original languageEnglish
Pages (from-to)1101-1109
Number of pages9
JournalInternational Journal of Medical Sciences
Volume14
Issue number11
DOIs
Publication statusPublished - 2017 Sep 4

Fingerprint

Plasma Gases
Gold
Nanoparticles
Melanoma
Antibodies
Focal Adhesion Protein-Tyrosine Kinases
Keratinocytes
Apoptosis
Plasma Cells
Cell Survival

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Choi, Byul Bo Ra ; Choi, Jeong Hae ; Hong, Jin Woo ; Song, Ki Won ; Lee, Hae June ; Kim, Uk Kyu ; Kim, Gyoo Cheon. / Selective killing of melanoma cells with non-thermal atmospheric pressure plasma and p-FAK antibody conjugated gold nanoparticles. In: International Journal of Medical Sciences. 2017 ; Vol. 14, No. 11. pp. 1101-1109.
@article{ad79c874ad724f60a03611d8e71d496c,
title = "Selective killing of melanoma cells with non-thermal atmospheric pressure plasma and p-FAK antibody conjugated gold nanoparticles",
abstract = "Melanomas are fast growing high-mortality tumors, and specific treatments for melanomas are needed. Melanoma cells overexpress focal adhesion kinase (FAK) compared to normal keratinocytes, and we sought to exploit this difference to create a selectively lethal therapy. We combined gold nanoparticles (GNP) with antibodies targeting phosphorylated FAK (p-FAK). These conjugates (p-FAK-GNP) entered G361 melanoma cells and bound p-FAK. Treatment with p-FAK-GNP decreased the viability of G361 cells in a time dependent manner by inducing apoptosis. To maximize the preferential killing of G361 cells, non-thermal atmospheric pressure plasma was used to stimulate the GNP within p-FAK-GNP. Combined treatment with plasma and p-FAK-GNP showed much higher lethality against G361 cells than HaCaT keratinocyte cells. The p-FAK-GNP induced apoptosis over 48 hours in G361 cells, whereas plasma and p-FAK-GNP killed G361 cells immediately. This study demonstrates that combining plasma with p-FAK-GNP results in selective lethality against human melanoma cells.",
author = "Choi, {Byul Bo Ra} and Choi, {Jeong Hae} and Hong, {Jin Woo} and Song, {Ki Won} and Lee, {Hae June} and Kim, {Uk Kyu} and Kim, {Gyoo Cheon}",
year = "2017",
month = "9",
day = "4",
doi = "10.7150/ijms.20104",
language = "English",
volume = "14",
pages = "1101--1109",
journal = "International Journal of Medical Sciences",
issn = "1449-1907",
publisher = "Ivyspring International Publisher",
number = "11",

}

Selective killing of melanoma cells with non-thermal atmospheric pressure plasma and p-FAK antibody conjugated gold nanoparticles. / Choi, Byul Bo Ra; Choi, Jeong Hae; Hong, Jin Woo; Song, Ki Won; Lee, Hae June; Kim, Uk Kyu; Kim, Gyoo Cheon.

In: International Journal of Medical Sciences, Vol. 14, No. 11, 04.09.2017, p. 1101-1109.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selective killing of melanoma cells with non-thermal atmospheric pressure plasma and p-FAK antibody conjugated gold nanoparticles

AU - Choi, Byul Bo Ra

AU - Choi, Jeong Hae

AU - Hong, Jin Woo

AU - Song, Ki Won

AU - Lee, Hae June

AU - Kim, Uk Kyu

AU - Kim, Gyoo Cheon

PY - 2017/9/4

Y1 - 2017/9/4

N2 - Melanomas are fast growing high-mortality tumors, and specific treatments for melanomas are needed. Melanoma cells overexpress focal adhesion kinase (FAK) compared to normal keratinocytes, and we sought to exploit this difference to create a selectively lethal therapy. We combined gold nanoparticles (GNP) with antibodies targeting phosphorylated FAK (p-FAK). These conjugates (p-FAK-GNP) entered G361 melanoma cells and bound p-FAK. Treatment with p-FAK-GNP decreased the viability of G361 cells in a time dependent manner by inducing apoptosis. To maximize the preferential killing of G361 cells, non-thermal atmospheric pressure plasma was used to stimulate the GNP within p-FAK-GNP. Combined treatment with plasma and p-FAK-GNP showed much higher lethality against G361 cells than HaCaT keratinocyte cells. The p-FAK-GNP induced apoptosis over 48 hours in G361 cells, whereas plasma and p-FAK-GNP killed G361 cells immediately. This study demonstrates that combining plasma with p-FAK-GNP results in selective lethality against human melanoma cells.

AB - Melanomas are fast growing high-mortality tumors, and specific treatments for melanomas are needed. Melanoma cells overexpress focal adhesion kinase (FAK) compared to normal keratinocytes, and we sought to exploit this difference to create a selectively lethal therapy. We combined gold nanoparticles (GNP) with antibodies targeting phosphorylated FAK (p-FAK). These conjugates (p-FAK-GNP) entered G361 melanoma cells and bound p-FAK. Treatment with p-FAK-GNP decreased the viability of G361 cells in a time dependent manner by inducing apoptosis. To maximize the preferential killing of G361 cells, non-thermal atmospheric pressure plasma was used to stimulate the GNP within p-FAK-GNP. Combined treatment with plasma and p-FAK-GNP showed much higher lethality against G361 cells than HaCaT keratinocyte cells. The p-FAK-GNP induced apoptosis over 48 hours in G361 cells, whereas plasma and p-FAK-GNP killed G361 cells immediately. This study demonstrates that combining plasma with p-FAK-GNP results in selective lethality against human melanoma cells.

UR - http://www.scopus.com/inward/record.url?scp=85029648882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029648882&partnerID=8YFLogxK

U2 - 10.7150/ijms.20104

DO - 10.7150/ijms.20104

M3 - Article

C2 - 29104464

AN - SCOPUS:85029648882

VL - 14

SP - 1101

EP - 1109

JO - International Journal of Medical Sciences

JF - International Journal of Medical Sciences

SN - 1449-1907

IS - 11

ER -