Selective medium for isolation of Bacteroides gracilis

K. Lee, E. J. Baron, P. Summanen, S. M. Finegold

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Abstract

A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium fumarate, and potassium nitrate. All 18 strains of B. gracilis tested grew with only minimal inhibition. Most of the other 214 organisms tested, including most Bacteroides species, other anaerobes, and a substantial number of facultative anaerobes, were significantly inhibited by the medium. In a diagnostic study of 49 clinical, specimens (28 patients with intra-abdominal infection, mostly gangrenous or perforated appendicitis), four strains of B. gracilis were isolated (from 4 different patients) on B. gracilis selective agar but were not detected on standard media.

Original languageEnglish
Pages (from-to)1747-1750
Number of pages4
JournalJournal of Clinical Microbiology
Volume28
Issue number8
Publication statusPublished - 1990 Aug 6

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All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Lee, K., Baron, E. J., Summanen, P., & Finegold, S. M. (1990). Selective medium for isolation of Bacteroides gracilis. Journal of Clinical Microbiology, 28(8), 1747-1750.