Abstract
A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium fumarate, and potassium nitrate. All 18 strains of B. gracilis tested grew with only minimal inhibition. Most of the other 214 organisms tested, including most Bacteroides species, other anaerobes, and a substantial number of facultative anaerobes, were significantly inhibited by the medium. In a diagnostic study of 49 clinical, specimens (28 patients with intra-abdominal infection, mostly gangrenous or perforated appendicitis), four strains of B. gracilis were isolated (from 4 different patients) on B. gracilis selective agar but were not detected on standard media.
| Original language | English |
|---|---|
| Pages (from-to) | 1747-1750 |
| Number of pages | 4 |
| Journal | Journal of Clinical Microbiology |
| Volume | 28 |
| Issue number | 8 |
| Publication status | Published - 1990 Aug 6 |
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All Science Journal Classification (ASJC) codes
- Microbiology (medical)
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Selective medium for isolation of Bacteroides gracilis. / Lee, K.; Baron, E. J.; Summanen, P.; Finegold, S. M.
In: Journal of Clinical Microbiology, Vol. 28, No. 8, 06.08.1990, p. 1747-1750.Research output: Contribution to journal › Article
TY - JOUR
T1 - Selective medium for isolation of Bacteroides gracilis
AU - Lee, K.
AU - Baron, E. J.
AU - Summanen, P.
AU - Finegold, S. M.
PY - 1990/8/6
Y1 - 1990/8/6
N2 - A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium fumarate, and potassium nitrate. All 18 strains of B. gracilis tested grew with only minimal inhibition. Most of the other 214 organisms tested, including most Bacteroides species, other anaerobes, and a substantial number of facultative anaerobes, were significantly inhibited by the medium. In a diagnostic study of 49 clinical, specimens (28 patients with intra-abdominal infection, mostly gangrenous or perforated appendicitis), four strains of B. gracilis were isolated (from 4 different patients) on B. gracilis selective agar but were not detected on standard media.
AB - A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium fumarate, and potassium nitrate. All 18 strains of B. gracilis tested grew with only minimal inhibition. Most of the other 214 organisms tested, including most Bacteroides species, other anaerobes, and a substantial number of facultative anaerobes, were significantly inhibited by the medium. In a diagnostic study of 49 clinical, specimens (28 patients with intra-abdominal infection, mostly gangrenous or perforated appendicitis), four strains of B. gracilis were isolated (from 4 different patients) on B. gracilis selective agar but were not detected on standard media.
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M3 - Article
C2 - 2144297
AN - SCOPUS:0025322285
VL - 28
SP - 1747
EP - 1750
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 8
ER -