Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and -2

Krishna P. Bhat, Christopher E. Pelloski, Yujian Zhang, Se Hoon Kim, Clarissa deLaCruz, Michael Rehli, Kenneth D. Aldape

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-α suppresses YKL-40 expression in glioma cell lines in a nuclear factor κB (NF-κB) dependent manner. Even though TNF-α causes recruitment of p65 and p50 subunits of NF-κB to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-κB regulation of one of its targets in a strict cell type specific manner.

Original languageEnglish
Pages (from-to)3193-3200
Number of pages8
JournalFEBS Letters
Volume582
Issue number21-22
DOIs
Publication statusPublished - 2008 Sep 22

Fingerprint

Histone Deacetylase 2
Histone Deacetylase 1
Glioma
Tumor Necrosis Factor-alpha
Cells
Cell Line
Histone Deacetylases
Histones
Chromatin
Assays
Chromatin Immunoprecipitation
Glioblastoma
Tissue
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Bhat, Krishna P. ; Pelloski, Christopher E. ; Zhang, Yujian ; Kim, Se Hoon ; deLaCruz, Clarissa ; Rehli, Michael ; Aldape, Kenneth D. / Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and -2. In: FEBS Letters. 2008 ; Vol. 582, No. 21-22. pp. 3193-3200.
@article{272cee95953e43468533436a45f07f50,
title = "Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and -2",
abstract = "Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-α suppresses YKL-40 expression in glioma cell lines in a nuclear factor κB (NF-κB) dependent manner. Even though TNF-α causes recruitment of p65 and p50 subunits of NF-κB to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-κB regulation of one of its targets in a strict cell type specific manner.",
author = "Bhat, {Krishna P.} and Pelloski, {Christopher E.} and Yujian Zhang and Kim, {Se Hoon} and Clarissa deLaCruz and Michael Rehli and Aldape, {Kenneth D.}",
year = "2008",
month = "9",
day = "22",
doi = "10.1016/j.febslet.2008.08.010",
language = "English",
volume = "582",
pages = "3193--3200",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "21-22",

}

Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and -2. / Bhat, Krishna P.; Pelloski, Christopher E.; Zhang, Yujian; Kim, Se Hoon; deLaCruz, Clarissa; Rehli, Michael; Aldape, Kenneth D.

In: FEBS Letters, Vol. 582, No. 21-22, 22.09.2008, p. 3193-3200.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and -2

AU - Bhat, Krishna P.

AU - Pelloski, Christopher E.

AU - Zhang, Yujian

AU - Kim, Se Hoon

AU - deLaCruz, Clarissa

AU - Rehli, Michael

AU - Aldape, Kenneth D.

PY - 2008/9/22

Y1 - 2008/9/22

N2 - Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-α suppresses YKL-40 expression in glioma cell lines in a nuclear factor κB (NF-κB) dependent manner. Even though TNF-α causes recruitment of p65 and p50 subunits of NF-κB to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-κB regulation of one of its targets in a strict cell type specific manner.

AB - Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-α suppresses YKL-40 expression in glioma cell lines in a nuclear factor κB (NF-κB) dependent manner. Even though TNF-α causes recruitment of p65 and p50 subunits of NF-κB to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-κB regulation of one of its targets in a strict cell type specific manner.

UR - http://www.scopus.com/inward/record.url?scp=51249101290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51249101290&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2008.08.010

DO - 10.1016/j.febslet.2008.08.010

M3 - Article

C2 - 18708058

AN - SCOPUS:51249101290

VL - 582

SP - 3193

EP - 3200

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 21-22

ER -