Abstract
Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-α suppresses YKL-40 expression in glioma cell lines in a nuclear factor κB (NF-κB) dependent manner. Even though TNF-α causes recruitment of p65 and p50 subunits of NF-κB to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-κB regulation of one of its targets in a strict cell type specific manner.
| Original language | English |
|---|---|
| Pages (from-to) | 3193-3200 |
| Number of pages | 8 |
| Journal | FEBS Letters |
| Volume | 582 |
| Issue number | 21-22 |
| DOIs | |
| Publication status | Published - 2008 Sep 22 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and -2. / Bhat, Krishna P.; Pelloski, Christopher E.; Zhang, Yujian; Kim, Se Hoon; deLaCruz, Clarissa; Rehli, Michael; Aldape, Kenneth D.
In: FEBS Letters, Vol. 582, No. 21-22, 22.09.2008, p. 3193-3200.Research output: Contribution to journal › Article
TY - JOUR
T1 - Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and -2
AU - Bhat, Krishna P.
AU - Pelloski, Christopher E.
AU - Zhang, Yujian
AU - Kim, Se Hoon
AU - deLaCruz, Clarissa
AU - Rehli, Michael
AU - Aldape, Kenneth D.
PY - 2008/9/22
Y1 - 2008/9/22
N2 - Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-α suppresses YKL-40 expression in glioma cell lines in a nuclear factor κB (NF-κB) dependent manner. Even though TNF-α causes recruitment of p65 and p50 subunits of NF-κB to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-κB regulation of one of its targets in a strict cell type specific manner.
AB - Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-α suppresses YKL-40 expression in glioma cell lines in a nuclear factor κB (NF-κB) dependent manner. Even though TNF-α causes recruitment of p65 and p50 subunits of NF-κB to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-κB regulation of one of its targets in a strict cell type specific manner.
UR - http://www.scopus.com/inward/record.url?scp=51249101290&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51249101290&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2008.08.010
DO - 10.1016/j.febslet.2008.08.010
M3 - Article
C2 - 18708058
AN - SCOPUS:51249101290
VL - 582
SP - 3193
EP - 3200
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 21-22
ER -