Abstract
Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 224-226 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 Jan 1 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 15/10/25 → 15/10/29 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
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Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays. / Chen, Yu Chih; Baac, Hyoung Won; Lee, Kyu Tae; Teichert, Kendall; Hart, A. John; Guo, L. Jay; Yoon, Euisik.
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 224-226 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays
AU - Chen, Yu Chih
AU - Baac, Hyoung Won
AU - Lee, Kyu Tae
AU - Teichert, Kendall
AU - Hart, A. John
AU - Guo, L. Jay
AU - Yoon, Euisik
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells.
AB - Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells.
UR - http://www.scopus.com/inward/record.url?scp=84983354517&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983354517&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983354517
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 224
EP - 226
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -