We reportanovel approach for generating nanosized DNA hollow spheres (HSs) using enzymatically produced DNA microsponges in aself-templating manner. Inprevious studies, preparation of DNA nanostructures with specified functions required multiple complicated steps. In this study, however, a simple treatment with the nucleophilic agent 4-dimethylaminopyridine (DMAP) enabledagradual disentanglement of DNA in microsponges byelectrostatic interactions between DMAP and DNA, and the DNA underwent areassembly processto generate hollow shell structures without denaturation/annealing by thermal cycling. In addition, this synthetic process was conducted in a water-based system without organic solvents, enabling the synthesis of biologically and environmentally friendly products. Based on the benefits of hollow shell structures, which include their high surface-to-volume ratio and ability to encapsulate small molecules, we envision that this simple approach for synthesizing DNA HSs will provideanew platform for maximizing their potential useindrug delivery and bio-imaging.
Bibliographical noteFunding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1E1A1A01074343 and NRF-2017R1E1A1A01075027). And this research was also supported Creative Materials Discovery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) (NRF-2017M3D1A1039423 and NRF-2017M3D1A1039422).
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All Science Journal Classification (ASJC) codes
- Biomedical Engineering