We fabricated multimode nanoprobes for acquisition of biological information at different object levels, i.e., in vivo detection and ex vivo validation for characterizing tumor angiogenesis. Fluorescent magnetic nanoprobes (FMNPs) were synthesized by using amphiphilic pyrenyl polyethylene glycol (Py-PEG) and superparamagnetic MnFe2O4 nanocrystals (MNCs). Py-PEG, which is synthesized by conjugation of hydrophilic PEG with hydrophobic and fluorescent 1-pyrenebutyric acid through an esterification process, is capable of self-assembly and maintaining a high UV fluorescent intensity in aqueous phase. Py-PEG can be used as a fluorescent surfactant that simultaneously and efficiently encapsulates MNCs to exhibit fluorescent and magnetic properties as well as maintaining high water-solubility. Consequently, we proved that our biologically non-toxic FMNPs were prominent multimode imaging probes by showing not only excellent MR sensitivity but also high illumination intensity with strong signal strength under short exposure time of UV light from the extensive imaging studies of in vitro/vivo and ex vivo using orthotopic and xenograft mice models.
|Number of pages||10|
|Publication status||Published - 2010 Dec|
Bibliographical noteFunding Information:
This study was supported by a grant of the Korea Health 21 R&D Project , Ministry of Health & Welfare, Republic of Korea ( A085136 ) and the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (No. 2009-0084190 ).
All Science Journal Classification (ASJC) codes
- Ceramics and Composites
- Mechanics of Materials