Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA: Noncovalent strategy in developing a gene delivery carrier

Yong Beom Lim, Taeil Kim, Jae Wook Lee, Seon Mi Kim, Hee Joon Kim, Kimoon Kim, Jong Sang Park

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH2NH2+(CH2) 4NH3+]z], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.

Original languageEnglish
Pages (from-to)1181-1185
Number of pages5
JournalBioconjugate Chemistry
Volume13
Issue number6
DOIs
Publication statusPublished - 2002 Nov 1

Fingerprint

Dendrimers
DNA
Genes
Dynamic light scattering
Cytotoxicity
Fluorescence
Cells
Putrescine
cucurbituril

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Lim, Yong Beom ; Kim, Taeil ; Lee, Jae Wook ; Kim, Seon Mi ; Kim, Hee Joon ; Kim, Kimoon ; Park, Jong Sang. / Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA : Noncovalent strategy in developing a gene delivery carrier. In: Bioconjugate Chemistry. 2002 ; Vol. 13, No. 6. pp. 1181-1185.
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Self-assembled ternary complex of cationic dendrimer, cucurbituril, and DNA : Noncovalent strategy in developing a gene delivery carrier. / Lim, Yong Beom; Kim, Taeil; Lee, Jae Wook; Kim, Seon Mi; Kim, Hee Joon; Kim, Kimoon; Park, Jong Sang.

In: Bioconjugate Chemistry, Vol. 13, No. 6, 01.11.2002, p. 1181-1185.

Research output: Contribution to journalArticle

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