Self-doped conjugated polymeric nanoassembly by simplified process for optical cancer theragnosis

Jeonghun Kim, Eugene Lee, Yoochan Hong, Byeonggwan Kim, Minhee Ku, Dan Heo, Jihye Choi, Jongbeom Na, Jungmok You, Seungjoo Haam, yongmin Huh, Jinsuck Suh, Eunkyoung Kim, Jaemoon Yang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To access smart optical theragnosis for cancer, an easily processable heterocyclic conjugated polymer (poly(sodium3-((3-methyl-3,4-dihydro-2H-thieno[3,4-b][1,4]dioxepin-3-yl)methoxy)propane-1-sulfonate), PPDS) nanoassembly is fabricated by a surfactant-free one-step process, without the laborious ordinary multicoating process. The conjugated nanoassembly, with a self-doped structure, provides strong absorbance in the near-infrared (NIR) range even in a neutral pH medium and exhibits excellent stability (>six months). In addition, the prepared PPDS nanoassembly shows a high photothermal conversion efficiency of 31.4% in organic photothermal nanoparticles. In particular, the PPDS nanoassembly is stably suspended in the biological medium without any additives. Through a simple immobilization with the anti-CD44 antibody, the prepared biomarker-targetable PPDS nanoassembly demonstrates specific targeting toward CD44 (expressed in stem-like cancer cells), allowing NIR absorbance imaging and the efficient targeted photothermal damaging of CD44-expressing cancer cells, from in vitro 3D mammospheres (similar to the practical structure of tumor in the body) to in vivo xenograft mice tumor models (breast cancer and fibrosarcoma). In this study, the most simplified preparation method is for this organic conjugated polymer-based nanoassembly by a molecular approach is reported, and demonstrated as a highly promising optical nanoagent for optical cancer theragnosis. A thiophene-based photothermal (PT) organic nanoprobe is synthesized, with a simplified preparation process not requiring PEGylation or multicoating. The synthesized nanoassembly shows good stability, biocompatibility, and PT properties. The nanoassembly is applied to 3D tumor mammospheres and breast cancer, and shows excellent specific targeting of the CD44-expressing cancerous cells, near-infrared (NIR) absorbance imaging in vivo, and effective damaging by NIR light irradiation.

Original languageEnglish
Pages (from-to)2260-2269
Number of pages10
JournalAdvanced Functional Materials
Volume25
Issue number15
DOIs
Publication statusPublished - 2015 Apr 15

Fingerprint

Tumors
cancer
Conjugated polymers
Infrared imaging
Cells
Nanoprobes
Infrared radiation
Thiophenes
Propane
Organic polymers
tumors
Biomarkers
Thiophene
Biocompatibility
Heterografts
Antibodies
Surface-Active Agents
breast
Conversion efficiency
Surface active agents

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrochemistry

Cite this

Kim, Jeonghun ; Lee, Eugene ; Hong, Yoochan ; Kim, Byeonggwan ; Ku, Minhee ; Heo, Dan ; Choi, Jihye ; Na, Jongbeom ; You, Jungmok ; Haam, Seungjoo ; Huh, yongmin ; Suh, Jinsuck ; Kim, Eunkyoung ; Yang, Jaemoon. / Self-doped conjugated polymeric nanoassembly by simplified process for optical cancer theragnosis. In: Advanced Functional Materials. 2015 ; Vol. 25, No. 15. pp. 2260-2269.
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Self-doped conjugated polymeric nanoassembly by simplified process for optical cancer theragnosis. / Kim, Jeonghun; Lee, Eugene; Hong, Yoochan; Kim, Byeonggwan; Ku, Minhee; Heo, Dan; Choi, Jihye; Na, Jongbeom; You, Jungmok; Haam, Seungjoo; Huh, yongmin; Suh, Jinsuck; Kim, Eunkyoung; Yang, Jaemoon.

In: Advanced Functional Materials, Vol. 25, No. 15, 15.04.2015, p. 2260-2269.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Self-doped conjugated polymeric nanoassembly by simplified process for optical cancer theragnosis

AU - Kim, Jeonghun

AU - Lee, Eugene

AU - Hong, Yoochan

AU - Kim, Byeonggwan

AU - Ku, Minhee

AU - Heo, Dan

AU - Choi, Jihye

AU - Na, Jongbeom

AU - You, Jungmok

AU - Haam, Seungjoo

AU - Huh, yongmin

AU - Suh, Jinsuck

AU - Kim, Eunkyoung

AU - Yang, Jaemoon

PY - 2015/4/15

Y1 - 2015/4/15

N2 - To access smart optical theragnosis for cancer, an easily processable heterocyclic conjugated polymer (poly(sodium3-((3-methyl-3,4-dihydro-2H-thieno[3,4-b][1,4]dioxepin-3-yl)methoxy)propane-1-sulfonate), PPDS) nanoassembly is fabricated by a surfactant-free one-step process, without the laborious ordinary multicoating process. The conjugated nanoassembly, with a self-doped structure, provides strong absorbance in the near-infrared (NIR) range even in a neutral pH medium and exhibits excellent stability (>six months). In addition, the prepared PPDS nanoassembly shows a high photothermal conversion efficiency of 31.4% in organic photothermal nanoparticles. In particular, the PPDS nanoassembly is stably suspended in the biological medium without any additives. Through a simple immobilization with the anti-CD44 antibody, the prepared biomarker-targetable PPDS nanoassembly demonstrates specific targeting toward CD44 (expressed in stem-like cancer cells), allowing NIR absorbance imaging and the efficient targeted photothermal damaging of CD44-expressing cancer cells, from in vitro 3D mammospheres (similar to the practical structure of tumor in the body) to in vivo xenograft mice tumor models (breast cancer and fibrosarcoma). In this study, the most simplified preparation method is for this organic conjugated polymer-based nanoassembly by a molecular approach is reported, and demonstrated as a highly promising optical nanoagent for optical cancer theragnosis. A thiophene-based photothermal (PT) organic nanoprobe is synthesized, with a simplified preparation process not requiring PEGylation or multicoating. The synthesized nanoassembly shows good stability, biocompatibility, and PT properties. The nanoassembly is applied to 3D tumor mammospheres and breast cancer, and shows excellent specific targeting of the CD44-expressing cancerous cells, near-infrared (NIR) absorbance imaging in vivo, and effective damaging by NIR light irradiation.

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