Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans

Jong Won Yun; Kyung Hoon Jung; Jong Won Oh; Jae Heung Lee

doi:10.1007/BF02920254

Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans

Jong Won Yun, Kyung Hoon Jung, Jong Won Oh, Jae Heung Lee

Research output: Contribution to journal › Article

57 Citations (Scopus)

Abstract

Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2% calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells.

Original language	English
Pages (from-to)	299-308
Number of pages	10
Journal	Applied Biochemistry and Biotechnology
Volume	24-25
Issue number	1
DOIs	https://doi.org/10.1007/BF02920254
Publication status	Published - 1990 Mar 1

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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Yun, J. W., Jung, K. H., Oh, J. W., & Lee, J. H. (1990). Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans. Applied Biochemistry and Biotechnology, 24-25(1), 299-308. https://doi.org/10.1007/BF02920254

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abstract = "Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2% calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells.",

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