Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans

Jong Won Yun, Kyung Hoon Jung, Jong Won Oh, Jae Heung Lee

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2% calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells.

Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalApplied Biochemistry and Biotechnology
Volume24-25
Issue number1
DOIs
Publication statusPublished - 1990 Mar 1

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Immobilized Cells
Oligosaccharides
Sugar (sucrose)
Sucrose
Alginate
Cells
inulosucrase
Cell-Free System
Bioreactors
Calcium
Chemical analysis
pullulan
alginic acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

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abstract = "Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2{\%} calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells.",
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Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans. / Yun, Jong Won; Jung, Kyung Hoon; Oh, Jong Won; Lee, Jae Heung.

In: Applied Biochemistry and Biotechnology, Vol. 24-25, No. 1, 01.03.1990, p. 299-308.

Research output: Contribution to journalArticle

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