Abstract
Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2% calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells.
| Original language | English |
|---|---|
| Pages (from-to) | 299-308 |
| Number of pages | 10 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 24-25 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1990 Mar 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
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Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans. / Yun, Jong Won; Jung, Kyung Hoon; Oh, Jong Won; Lee, Jae Heung.
In: Applied Biochemistry and Biotechnology, Vol. 24-25, No. 1, 01.03.1990, p. 299-308.Research output: Contribution to journal › Article
TY - JOUR
T1 - Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans
AU - Yun, Jong Won
AU - Jung, Kyung Hoon
AU - Oh, Jong Won
AU - Lee, Jae Heung
PY - 1990/3/1
Y1 - 1990/3/1
N2 - Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2% calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells.
AB - Aureobasidium pullulans cells with fructosyltransferase activity were immobilized in 2% calcium alginate beads, and the production of fructo-oligosaccharides from sucrose was studied in a stirred tank bioreactor. It was found that cells of A. pullulans were entrapped evenly on the alginate matrix of 2.2 mm in diameter, and an effectiveness factor of the beads was determined to be 0.3. By comparison with the system of free cells in batch operation, the total amount of fructo-oligosaccharides produced by immobilized cells was similar although the composition of fructo-oligosaccharides was found to be different. In semibatch operation with immobilized cells, reproducible results up to 60 cycles were obtained at 50 °C and this operation resulted in no loss of activity of immobilized cells.
UR - http://www.scopus.com/inward/record.url?scp=0025040927&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025040927&partnerID=8YFLogxK
U2 - 10.1007/BF02920254
DO - 10.1007/BF02920254
M3 - Article
AN - SCOPUS:0025040927
VL - 24-25
SP - 299
EP - 308
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
SN - 0273-2289
IS - 1
ER -