Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array

Jaehyung Lee, Min Ho Hong, Sanghun Han, Jukwan Na, Ilsoo Kim, Yong Joon Kwon, Yong-beom Lim, Heon-Jin Choi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.

Original languageEnglish
Article number341
JournalNanoscale Research Letters
Volume11
Issue number1
DOIs
Publication statusPublished - 2016 Dec 1

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human immunodeficiency virus
Response Elements
Silicon
RNA
Nanowires
nanowires
Electrodes
electrodes
silicon
Voltammetry
Peptides
peptides
pulses
linearity
Ions
selectivity
sensitivity
ions

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Lee, Jaehyung ; Hong, Min Ho ; Han, Sanghun ; Na, Jukwan ; Kim, Ilsoo ; Kwon, Yong Joon ; Lim, Yong-beom ; Choi, Heon-Jin. / Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array. In: Nanoscale Research Letters. 2016 ; Vol. 11, No. 1.
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abstract = "In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.",
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Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array. / Lee, Jaehyung; Hong, Min Ho; Han, Sanghun; Na, Jukwan; Kim, Ilsoo; Kwon, Yong Joon; Lim, Yong-beom; Choi, Heon-Jin.

In: Nanoscale Research Letters, Vol. 11, No. 1, 341, 01.12.2016.

Research output: Contribution to journalArticle

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AU - Lee, Jaehyung

AU - Hong, Min Ho

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AU - Kim, Ilsoo

AU - Kwon, Yong Joon

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AU - Choi, Heon-Jin

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