Abstract
In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.
| Original language | English |
|---|---|
| Article number | 341 |
| Journal | Nanoscale Research Letters |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2016 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
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Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array. / Lee, Jaehyung; Hong, Min Ho; Han, Sanghun; Na, Jukwan; Kim, Ilsoo; Kwon, Yong Joon; Lim, Yong-beom; Choi, Heon-Jin.
In: Nanoscale Research Letters, Vol. 11, No. 1, 341, 01.12.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array
AU - Lee, Jaehyung
AU - Hong, Min Ho
AU - Han, Sanghun
AU - Na, Jukwan
AU - Kim, Ilsoo
AU - Kwon, Yong Joon
AU - Lim, Yong-beom
AU - Choi, Heon-Jin
PY - 2016/12/1
Y1 - 2016/12/1
N2 - In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.
AB - In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.
UR - http://www.scopus.com/inward/record.url?scp=84979224607&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84979224607&partnerID=8YFLogxK
U2 - 10.1186/s11671-016-1504-8
DO - 10.1186/s11671-016-1504-8
M3 - Article
AN - SCOPUS:84979224607
VL - 11
JO - Nanoscale Research Letters
JF - Nanoscale Research Letters
SN - 1931-7573
IS - 1
M1 - 341
ER -