Sensitive Plasmonic Detection of miR-10b in Biological Samples Using Enzyme-Assisted Target Recycling and Developed LSPR Probe

Jisun Ki, Hyo Young Lee, Hye Young Son, yongmin Huh, Seungjoo Haam

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A portable and nonlabeled plasmonic biosensor was advanced to enable the sensitive and selective detection of microRNA (miRNA) in a biological sample. miRNAs can act on several key cellular processes, including cell differentiation, cell cycle progression, and function as oncogenes. Detection of circulating miRNAs, especially in blood or urine samples, allows noninvasive and simple diagnosis of diseases. Herein, we report a localized surface plasmon resonance sensor (LSPR) based on an enzyme-assisted target recycling system and a developed LSPR probe for the detection of gastric cancer relevant miRNAs, miR-10b. The sensitivity of the sensor was improved by increasing the concentration of the signal-amplifying agent using the duplex-specific nuclease and by strongly binding the developed LSPR probe, tannic acid capping gold nanoparticles, to the DNA. Under optimal conditions, miR-10b detection could be realized in the range of 5 pM-10 nM with a detection limit of 2.45 pM. This integrated detection system represents an approach to sensitive detection of miRNAs and offers great applications in personalized medicine and monitoring of cancer.

Original languageEnglish
Pages (from-to)18923-18929
Number of pages7
JournalACS Applied Materials and Interfaces
Volume11
Issue number21
DOIs
Publication statusPublished - 2019 May 29

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Surface plasmon resonance
MicroRNAs
Recycling
Enzymes
Sensors
Biosensors
Tannins
Medicine
DNA
Blood
Gold
Cells
Nanoparticles
Acids
Monitoring

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

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abstract = "A portable and nonlabeled plasmonic biosensor was advanced to enable the sensitive and selective detection of microRNA (miRNA) in a biological sample. miRNAs can act on several key cellular processes, including cell differentiation, cell cycle progression, and function as oncogenes. Detection of circulating miRNAs, especially in blood or urine samples, allows noninvasive and simple diagnosis of diseases. Herein, we report a localized surface plasmon resonance sensor (LSPR) based on an enzyme-assisted target recycling system and a developed LSPR probe for the detection of gastric cancer relevant miRNAs, miR-10b. The sensitivity of the sensor was improved by increasing the concentration of the signal-amplifying agent using the duplex-specific nuclease and by strongly binding the developed LSPR probe, tannic acid capping gold nanoparticles, to the DNA. Under optimal conditions, miR-10b detection could be realized in the range of 5 pM-10 nM with a detection limit of 2.45 pM. This integrated detection system represents an approach to sensitive detection of miRNAs and offers great applications in personalized medicine and monitoring of cancer.",
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Sensitive Plasmonic Detection of miR-10b in Biological Samples Using Enzyme-Assisted Target Recycling and Developed LSPR Probe. / Ki, Jisun; Lee, Hyo Young; Son, Hye Young; Huh, yongmin; Haam, Seungjoo.

In: ACS Applied Materials and Interfaces, Vol. 11, No. 21, 29.05.2019, p. 18923-18929.

Research output: Contribution to journalArticle

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