Separable roles of saccharomtces cerevsiae ste5 in gl cell cycle arrest and mating

K. Y. Choi, Y. J. Choi

Research output: Contribution to journalArticle

Abstract

M it ogen activated proteinf M APK t palhwav is an important net work for cell proliferation and differentiation. In budding yeast .V. nnntiat at lea M .six physiologically distinct MAPK cascades were identified. Among these, p heroin one mediated signaling pat h way is most well characterized and \} involved in both Gl cell tyck1 arn-4t and mating of t lie S. ctrrriiiat. Steî. to gether with Ste20, Stell and Ste7, is essential for the activation of the MAPK homolog. FuslJ. in pherornone induced signal t ransduct ion cascade in .V. /( ni'iniüf Steö physically links the protein kinases by forming the complex for t he regulation of the pheromone signaling pathway and may endow specificity fui the pathway. To understand the roles of Sten better we isolated Gl cell cycle .irrest xte-î mutants by chemical mut agenesis. Two of the tt ,r> mut ants( 1)24 XV and A-770} mate normally although it completely lost Gl cell cvcle arrest function. Therefore these t< > mutation,-, affects only t lie (II arrest function Furthermore, most of our cell cycle arres detective ,s/('"> mutants show dom inant negative phenotype when transforiüed into a wild typp $'TI'.\l>+ strain. However, all of the Gl arrest detective .sf" .> mutant-4 show recessive pheno l\pe for mating. These resiilth Miggest.s roles of Sleri in cell cycle arrest and mating arc separable. Biochemical .inalvsis of Gl arrest defective mutant-4 show o factor dependent StefvFus.i interaction may important for (l arrest phenotype and activation of His'l kinasc.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997 Dec 1

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Cell Cycle Checkpoints
Cell Cycle
Cells
Phenotype
mutants
Saccharomycetales
Ants
Pheromones
Heroin
Chemical activation
Protein Kinases
Cell Differentiation
cell cycle
Cell Proliferation
Cell proliferation
Ions
phenotype
Yeast
Mutation
cell differentiation

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

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abstract = "M it ogen activated proteinf M APK t palhwav is an important net work for cell proliferation and differentiation. In budding yeast .V. nnntiat at lea M .six physiologically distinct MAPK cascades were identified. Among these, p heroin one mediated signaling pat h way is most well characterized and \} involved in both Gl cell tyck1 arn-4t and mating of t lie S. ctrrriiiat. Ste{\^i}. to gether with Ste20, Stell and Ste7, is essential for the activation of the MAPK homolog. FuslJ. in pherornone induced signal t ransduct ion cascade in .V. /( ni'ini{\"u}f Ste{\"o} physically links the protein kinases by forming the complex for t he regulation of the pheromone signaling pathway and may endow specificity fui the pathway. To understand the roles of Sten better we isolated Gl cell cycle .irrest xte-{\^i} mutants by chemical mut agenesis. Two of the tt ,r> mut ants( 1)24 XV and A-770} mate normally although it completely lost Gl cell cvcle arrest function. Therefore these t< > mutation,-, affects only t lie (II arrest function Furthermore, most of our cell cycle arres detective ,s/('{"}> mutants show dom inant negative phenotype when transfori{\"u}ed into a wild typp $'TI'.\l>+ strain. However, all of the Gl arrest detective .sf{"} .> mutant-4 show recessive pheno l\pe for mating. These resiilth Miggest.s roles of Sleri in cell cycle arrest and mating arc separable. Biochemical .inalvsis of Gl arrest defective mutant-4 show o factor dependent StefvFus.i interaction may important for (l arrest phenotype and activation of His'l kinasc.",
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Separable roles of saccharomtces cerevsiae ste5 in gl cell cycle arrest and mating. / Choi, K. Y.; Choi, Y. J.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997.

Research output: Contribution to journalArticle

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N2 - M it ogen activated proteinf M APK t palhwav is an important net work for cell proliferation and differentiation. In budding yeast .V. nnntiat at lea M .six physiologically distinct MAPK cascades were identified. Among these, p heroin one mediated signaling pat h way is most well characterized and \} involved in both Gl cell tyck1 arn-4t and mating of t lie S. ctrrriiiat. Steî. to gether with Ste20, Stell and Ste7, is essential for the activation of the MAPK homolog. FuslJ. in pherornone induced signal t ransduct ion cascade in .V. /( ni'iniüf Steö physically links the protein kinases by forming the complex for t he regulation of the pheromone signaling pathway and may endow specificity fui the pathway. To understand the roles of Sten better we isolated Gl cell cycle .irrest xte-î mutants by chemical mut agenesis. Two of the tt ,r> mut ants( 1)24 XV and A-770} mate normally although it completely lost Gl cell cvcle arrest function. Therefore these t< > mutation,-, affects only t lie (II arrest function Furthermore, most of our cell cycle arres detective ,s/('"> mutants show dom inant negative phenotype when transforiüed into a wild typp $'TI'.\l>+ strain. However, all of the Gl arrest detective .sf" .> mutant-4 show recessive pheno l\pe for mating. These resiilth Miggest.s roles of Sleri in cell cycle arrest and mating arc separable. Biochemical .inalvsis of Gl arrest defective mutant-4 show o factor dependent StefvFus.i interaction may important for (l arrest phenotype and activation of His'l kinasc.

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