Separation of apoptotic cells using a microfluidic device

Hyun Seok Kim, Oh Taek Son, Kun Hong Kim, Sang Hyeob Kim, Sunglyul Maeng, Hyo il Jung

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A highly sensitive microfluidic device has been developed to separate apoptotic cells. Apoptotic Jurkat cells were selectively labeled with magnetic beads (0.8 μm diam) using the C2A protein which recognizes phosphatidylserine. The cell mixture was flowed through a microfluidic channel and apoptotic cells were separated by a 0.3 T permanent magnet. Separations using our device showed 96% agreement with those of a commercial flow cytometer, indicating our device can be used to sort apoptotic cells in a miniaturized system.

Original languageEnglish
Pages (from-to)1659-1663
Number of pages5
JournalBiotechnology Letters
Volume29
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

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Lab-On-A-Chip Devices
Cell Separation
Microfluidics
Cells
Phosphatidylserines
Equipment and Supplies
Permanent magnets
Jurkat Cells
Magnets
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Kim, Hyun Seok ; Son, Oh Taek ; Kim, Kun Hong ; Kim, Sang Hyeob ; Maeng, Sunglyul ; Jung, Hyo il. / Separation of apoptotic cells using a microfluidic device. In: Biotechnology Letters. 2007 ; Vol. 29, No. 11. pp. 1659-1663.
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Kim, HS, Son, OT, Kim, KH, Kim, SH, Maeng, S & Jung, HI 2007, 'Separation of apoptotic cells using a microfluidic device', Biotechnology Letters, vol. 29, no. 11, pp. 1659-1663. https://doi.org/10.1007/s10529-007-9451-1

Separation of apoptotic cells using a microfluidic device. / Kim, Hyun Seok; Son, Oh Taek; Kim, Kun Hong; Kim, Sang Hyeob; Maeng, Sunglyul; Jung, Hyo il.

In: Biotechnology Letters, Vol. 29, No. 11, 01.11.2007, p. 1659-1663.

Research output: Contribution to journalArticle

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