Abstract
The supercritical fluid extraction (SFE) behavior was investigated to extract astaxanthin from the red yeast Phaffia rhodozyma, which was disrupted and dried by bead mill and spray dryer, respectively. The effects of extraction pressure (102-500 bar), temperature (40, 60 and 80 °C), CO2 flow rate (superficial velocities of 0.27 and 0.54 cm/min) and the use of ethyl alcohol as a modifier (1, 5, 10, 15 vol.%) on the extraction efficiency were determined. The highest yield of carotenoids and astaxanthin with equal amounts of CO2 (50 g) was 84 and 90%, respectively, at 40 °C and 500 bar. Using a two-step pressure gradient operation, on changing the pressure from 300 to 500 bar, the concentration of astaxanthin in the second fraction at 500 bar increased by about 4 and 10 times at 40 and 60 °C, respectively, and the concentration of carotenoids increased by about 3.6 times at 40 °C and 13 times at 60 °C with the yield decreasing by about 40-50%.
| Original language | English |
|---|---|
| Pages (from-to) | 181-187 |
| Number of pages | 7 |
| Journal | Biochemical Engineering Journal |
| Volume | 11 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 2002 Sep 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Environmental Engineering
- Biomedical Engineering
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Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction. / Lim, Gio Bin; Lee, Sang Yun; Lee, Eun Kyu; Haam, Seungjoo; Kim, Woo Sik.
In: Biochemical Engineering Journal, Vol. 11, No. 2-3, 01.09.2002, p. 181-187.Research output: Contribution to journal › Article
TY - JOUR
T1 - Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction
AU - Lim, Gio Bin
AU - Lee, Sang Yun
AU - Lee, Eun Kyu
AU - Haam, Seungjoo
AU - Kim, Woo Sik
PY - 2002/9/1
Y1 - 2002/9/1
N2 - The supercritical fluid extraction (SFE) behavior was investigated to extract astaxanthin from the red yeast Phaffia rhodozyma, which was disrupted and dried by bead mill and spray dryer, respectively. The effects of extraction pressure (102-500 bar), temperature (40, 60 and 80 °C), CO2 flow rate (superficial velocities of 0.27 and 0.54 cm/min) and the use of ethyl alcohol as a modifier (1, 5, 10, 15 vol.%) on the extraction efficiency were determined. The highest yield of carotenoids and astaxanthin with equal amounts of CO2 (50 g) was 84 and 90%, respectively, at 40 °C and 500 bar. Using a two-step pressure gradient operation, on changing the pressure from 300 to 500 bar, the concentration of astaxanthin in the second fraction at 500 bar increased by about 4 and 10 times at 40 and 60 °C, respectively, and the concentration of carotenoids increased by about 3.6 times at 40 °C and 13 times at 60 °C with the yield decreasing by about 40-50%.
AB - The supercritical fluid extraction (SFE) behavior was investigated to extract astaxanthin from the red yeast Phaffia rhodozyma, which was disrupted and dried by bead mill and spray dryer, respectively. The effects of extraction pressure (102-500 bar), temperature (40, 60 and 80 °C), CO2 flow rate (superficial velocities of 0.27 and 0.54 cm/min) and the use of ethyl alcohol as a modifier (1, 5, 10, 15 vol.%) on the extraction efficiency were determined. The highest yield of carotenoids and astaxanthin with equal amounts of CO2 (50 g) was 84 and 90%, respectively, at 40 °C and 500 bar. Using a two-step pressure gradient operation, on changing the pressure from 300 to 500 bar, the concentration of astaxanthin in the second fraction at 500 bar increased by about 4 and 10 times at 40 and 60 °C, respectively, and the concentration of carotenoids increased by about 3.6 times at 40 °C and 13 times at 60 °C with the yield decreasing by about 40-50%.
UR - http://www.scopus.com/inward/record.url?scp=0036723049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036723049&partnerID=8YFLogxK
U2 - 10.1016/S1369-703X(02)00023-2
DO - 10.1016/S1369-703X(02)00023-2
M3 - Article
VL - 11
SP - 181
EP - 187
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
IS - 2-3
ER -