Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems

Soojeong Shin, Daeyoung Han, Min Chul Park, Ji Young Mun, Jonghoon Choi, Honggu Chun, Sunghoon Kim, Jong Wook Hong

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) are the cell-secreted nano- and micro-sized particles consisted of lipid bilayer containing nucleic acids and proteins for diagnosis and therapeutic applications. The inherent complexity of EVs is a source of heterogeneity in various potential applications of the biological nanovesicles including analysis. To diminish heterogeneity, EV should be isolated and separated according to their sizes and cargos. However, current technologies do not meet the requirements. We showed noninvasive and precise separation of EVs based on their sizes without any recognizable damages. We separated atto-liter volumes of biological nanoparticles through operation of the present system showing relatively large volume of sample treatment to milliliters within an hour. We observed distinct size and morphological differences of 30 to 100 nm of exosomes and apoptotic bodies through TEM analysis. Indeed, we confirmed the biological moiety variations through immunoblotting with noninvasively separated EVs opening new windows in study and application of the biological nanoparticles.

Original languageEnglish
Article number9907
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

Bibliographical note

Funding Information:
This research was supported by the Global Frontier Project Grant (NRF-2014M3A6A4062860, NRF-2013M3A6A4046061, NRF-M3A6A4-2010-0029785), Small Grant Exploratory Research (NRF-2014R1A1A2A160552910) and Engineering Research Center (2008-0061891) of the National Research Foundation, funded by the Ministry of Science, ICT & Future Planning of Korea.

All Science Journal Classification (ASJC) codes

  • General

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