Submicron microspheres were used directly without ligand coupling for the batch and continuous separations of proteins. In the batch experiments for separating BSA (bovine serum albumin) from BHb (bovine hemoglobin), introducing both hydrophobic effects for BSA and electrostatic repulsion for BHb (and vice versa) was required for high selectivity, and microspheres with low number density of surface groups were advantageous. For the continuous experiments, the utilization of a stirred cell was successful, where the microspheres were in the form of latex with good dispersion of particles. The flow rate without a pump was 0.5-1.3 ml min-1, and the ratio of BSA and BHb was varied. In the experiments for eliminating BHb from BSA, elution curves of BHb corresponded to the single component breakthrough curves, while those for BSA did not. The latter is believed to be due to the interference by BHb in the adsorption of BSA.
Bibliographical noteFunding Information:
This study was supported by a research grant from Bioproducts Research Center of Yonsei University (Project No. 94K3-1005-01-07-3).
All Science Journal Classification (ASJC) codes
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry