Separation of triacylglycerol species from interesterified oils by high-performance liquid chromatography

Jeung Hee Lee, Kerby C. Jones, Thomas A. Foglia, Alberto Nuñez, Jong Ho Lee, Yu Mi Kim, Phuong Lan Vu, Ki Teak Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Using a 1,3-regioselective lipase as a catalyst, soybean oil and olive oil were interesterified with the short-chain triacylglycerol tributyrin (1,2,3-tributyrylglycerol) to produce mixtures of structured triacylglycerols (SL-TAG). The SL-TAG were purified by column chromatography and analyzed by both normal-phase (silica column; NPSIL) and reversed-phase [octadecyl silane (ODS) column] high-performance liquid chromatography (HPLC). Individual SL-TAG molecular species were detected by evaporative light-scattering detection, and characterized by mass spectrometry. NPSIL HPLC successfully separated the newly synthesized SL-TAG into two groups of TAG: one composed of one butyryl group and two long-chain fatty acyl groups (from soybean or olive oil); the second was composed of two butyryl groups and one long-chain fatty acyl group. The SL-TAG species were further analyzed by reversed-phase HPLC which gave a more detailed separation of the TAG species present in the two SL-TAG.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalJAOCS, Journal of the American Oil Chemists' Society
Issue number3
Publication statusPublished - 2007 Mar

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a grant from the Korea Health 21 R and D Project, Ministry of Health and Welfare Republic of Korea (02-PJ1-PG1-CH15-0001).

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Organic Chemistry


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