Abstract
Using a 1,3-regioselective lipase as a catalyst, soybean oil and olive oil were interesterified with the short-chain triacylglycerol tributyrin (1,2,3-tributyrylglycerol) to produce mixtures of structured triacylglycerols (SL-TAG). The SL-TAG were purified by column chromatography and analyzed by both normal-phase (silica column; NPSIL) and reversed-phase [octadecyl silane (ODS) column] high-performance liquid chromatography (HPLC). Individual SL-TAG molecular species were detected by evaporative light-scattering detection, and characterized by mass spectrometry. NPSIL HPLC successfully separated the newly synthesized SL-TAG into two groups of TAG: one composed of one butyryl group and two long-chain fatty acyl groups (from soybean or olive oil); the second was composed of two butyryl groups and one long-chain fatty acyl group. The SL-TAG species were further analyzed by reversed-phase HPLC which gave a more detailed separation of the TAG species present in the two SL-TAG.
| Original language | English |
|---|---|
| Pages (from-to) | 211-217 |
| Number of pages | 7 |
| Journal | JAOCS, Journal of the American Oil Chemists' Society |
| Volume | 84 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2007 Mar |
Bibliographical note
Funding Information:Acknowledgments This work was supported by a grant from the Korea Health 21 R and D Project, Ministry of Health and Welfare Republic of Korea (02-PJ1-PG1-CH15-0001).
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)
- Organic Chemistry