Separation of triacylglycerol species from interesterified oils by high-performance liquid chromatography

Jeung Hee Lee, Kerby C. Jones, Thomas A. Foglia, Alberto Nuñez, Jong Ho Lee, Yu Mi Kim, Phuong Lan Vu, Ki Teak Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Using a 1,3-regioselective lipase as a catalyst, soybean oil and olive oil were interesterified with the short-chain triacylglycerol tributyrin (1,2,3-tributyrylglycerol) to produce mixtures of structured triacylglycerols (SL-TAG). The SL-TAG were purified by column chromatography and analyzed by both normal-phase (silica column; NPSIL) and reversed-phase [octadecyl silane (ODS) column] high-performance liquid chromatography (HPLC). Individual SL-TAG molecular species were detected by evaporative light-scattering detection, and characterized by mass spectrometry. NPSIL HPLC successfully separated the newly synthesized SL-TAG into two groups of TAG: one composed of one butyryl group and two long-chain fatty acyl groups (from soybean or olive oil); the second was composed of two butyryl groups and one long-chain fatty acyl group. The SL-TAG species were further analyzed by reversed-phase HPLC which gave a more detailed separation of the TAG species present in the two SL-TAG.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume84
Issue number3
DOIs
Publication statusPublished - 2007 Mar 1

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High performance liquid chromatography
Olive oil
Soybean oil
Oils
Soybean Oil
Triglycerides
Silanes
Column chromatography
Lipases
Lipase
Silicon Dioxide
Light scattering
Mass spectrometry
Silica
Catalysts
tributyrin
Olive Oil

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Organic Chemistry

Cite this

Lee, Jeung Hee ; Jones, Kerby C. ; Foglia, Thomas A. ; Nuñez, Alberto ; Lee, Jong Ho ; Kim, Yu Mi ; Vu, Phuong Lan ; Lee, Ki Teak. / Separation of triacylglycerol species from interesterified oils by high-performance liquid chromatography. In: JAOCS, Journal of the American Oil Chemists' Society. 2007 ; Vol. 84, No. 3. pp. 211-217.
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Separation of triacylglycerol species from interesterified oils by high-performance liquid chromatography. / Lee, Jeung Hee; Jones, Kerby C.; Foglia, Thomas A.; Nuñez, Alberto; Lee, Jong Ho; Kim, Yu Mi; Vu, Phuong Lan; Lee, Ki Teak.

In: JAOCS, Journal of the American Oil Chemists' Society, Vol. 84, No. 3, 01.03.2007, p. 211-217.

Research output: Contribution to journalArticle

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