Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates

Kyoung Yong Jeong, In Yong Lee, Taisoon Yong, Jae Hyun Lee, Eun Jin Kim, Joo Shil Lee, Chein Soo Hong, Jungwon Park

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17 Citations (Scopus)

Abstract

Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1. 0101, which appeared in 64. 0 % of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1. 0102 and 1. 0105 were predominant (58 %). Among the Der f 2 sequences, Der f 2. 0102 (40. 7 %) and a new variant with Gly at position 42 (27. 8 %) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26. 6 %); Val40, Thr49, and Asn114 (20. 0 %)) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalExperimental and Applied Acarology
Volume58
Issue number1
DOIs
Publication statusPublished - 2012 Sep 1

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dust mites
allergens
mite
polymorphism
amino acid
genetic polymorphism
dust
amino acid sequences
substitution
amino acid substitution
standardization
Dermatophagoides pteronyssinus
Dermatophagoides farinae
allergenicity
allergen
mites
clone
reverse transcriptase polymerase chain reaction
clones

All Science Journal Classification (ASJC) codes

  • Ecology
  • Insect Science

Cite this

Jeong, Kyoung Yong ; Lee, In Yong ; Yong, Taisoon ; Lee, Jae Hyun ; Kim, Eun Jin ; Lee, Joo Shil ; Hong, Chein Soo ; Park, Jungwon. / Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates. In: Experimental and Applied Acarology. 2012 ; Vol. 58, No. 1. pp. 35-42.
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abstract = "Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1. 0101, which appeared in 64. 0 {\%} of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1. 0102 and 1. 0105 were predominant (58 {\%}). Among the Der f 2 sequences, Der f 2. 0102 (40. 7 {\%}) and a new variant with Gly at position 42 (27. 8 {\%}) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26. 6 {\%}); Val40, Thr49, and Asn114 (20. 0 {\%})) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.",
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Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates. / Jeong, Kyoung Yong; Lee, In Yong; Yong, Taisoon; Lee, Jae Hyun; Kim, Eun Jin; Lee, Joo Shil; Hong, Chein Soo; Park, Jungwon.

In: Experimental and Applied Acarology, Vol. 58, No. 1, 01.09.2012, p. 35-42.

Research output: Contribution to journalArticle

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T1 - Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates

AU - Jeong, Kyoung Yong

AU - Lee, In Yong

AU - Yong, Taisoon

AU - Lee, Jae Hyun

AU - Kim, Eun Jin

AU - Lee, Joo Shil

AU - Hong, Chein Soo

AU - Park, Jungwon

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