Sequence polymorphisms of major German cockroach allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5

Kyoung Yong Jeong, Haeseok Lee, Kwang Hyun Shin, Myung Hee Yi, Kyoung Jin Jeong, Chein Soo Hong, Tai Soon Yong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: The allergenicity of allergens could be influenced by amino acid substitutions in B- or T-cell epitope regions. The German cockroach is known to produce potent allergens inducing strong IgE-mediated allergic reactions. This study was performed to investigate sequence variations in major allergens of the German cockroach. Methods: Reverse transcriptase PCR was used to amplify the cDNA sequences encoding major allergens of the German cockroach (Bla g 1, Bla g 2, Bla g 4, and Bla g 5). Results: The deduced amino acid sequences revealed 38 Bla g 1 variants with 1-7 amino acid substitutions (98.6-99.8% identity), 28 Bla g 2 variants with 1-3 substitutions (99.1-99.7%), 27 Bla g 4 variants with 0-32 substitutions (82.4-100%), and 8 Bla g 5 variants with 1-2 substitutions (99.0-99.5%), respectively. Bla g 1 and Bla g 2 showed sporadic amino acid substitutions despite the divergence in their sequences. Bla g 4 exhibited frequent variations, with clusters of substitutions in residues 29-38, 52-80, and 132-155. Sequence variations in Bla g 4 imply the presence of multiple isoforms and isoallergens, which may in turn have various effects on the IgE-binding capacity and T-cell responsiveness. Only 8 variants were found in Bla g 5, with infrequent amino acid changes of one or two residues. Conclusions: Analyses of T-cell and IgE-binding epitope regions would clarify the effect of sequence polymorphisms on allergenicity, which in turn will aid in the design of allergen formulations for diagnosis and immunotherapy for cockroach allergies.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalInternational Archives of Allergy and Immunology
Volume145
Issue number1
DOIs
Publication statusPublished - 2007 Dec 1

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Blattellidae
Allergens
Amino Acid Substitution
Immunoglobulin E
Hypersensitivity
T-Lymphocytes
Cockroaches
T-Lymphocyte Epitopes
Reverse Transcriptase Polymerase Chain Reaction
Immunotherapy
Epitopes
Amino Acid Sequence
Protein Isoforms
Complementary DNA
allergen Bla g 1
Amino Acids

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Jeong, Kyoung Yong ; Lee, Haeseok ; Shin, Kwang Hyun ; Yi, Myung Hee ; Jeong, Kyoung Jin ; Hong, Chein Soo ; Yong, Tai Soon. / Sequence polymorphisms of major German cockroach allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5. In: International Archives of Allergy and Immunology. 2007 ; Vol. 145, No. 1. pp. 1-8.
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title = "Sequence polymorphisms of major German cockroach allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5",
abstract = "Background: The allergenicity of allergens could be influenced by amino acid substitutions in B- or T-cell epitope regions. The German cockroach is known to produce potent allergens inducing strong IgE-mediated allergic reactions. This study was performed to investigate sequence variations in major allergens of the German cockroach. Methods: Reverse transcriptase PCR was used to amplify the cDNA sequences encoding major allergens of the German cockroach (Bla g 1, Bla g 2, Bla g 4, and Bla g 5). Results: The deduced amino acid sequences revealed 38 Bla g 1 variants with 1-7 amino acid substitutions (98.6-99.8{\%} identity), 28 Bla g 2 variants with 1-3 substitutions (99.1-99.7{\%}), 27 Bla g 4 variants with 0-32 substitutions (82.4-100{\%}), and 8 Bla g 5 variants with 1-2 substitutions (99.0-99.5{\%}), respectively. Bla g 1 and Bla g 2 showed sporadic amino acid substitutions despite the divergence in their sequences. Bla g 4 exhibited frequent variations, with clusters of substitutions in residues 29-38, 52-80, and 132-155. Sequence variations in Bla g 4 imply the presence of multiple isoforms and isoallergens, which may in turn have various effects on the IgE-binding capacity and T-cell responsiveness. Only 8 variants were found in Bla g 5, with infrequent amino acid changes of one or two residues. Conclusions: Analyses of T-cell and IgE-binding epitope regions would clarify the effect of sequence polymorphisms on allergenicity, which in turn will aid in the design of allergen formulations for diagnosis and immunotherapy for cockroach allergies.",
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Sequence polymorphisms of major German cockroach allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5. / Jeong, Kyoung Yong; Lee, Haeseok; Shin, Kwang Hyun; Yi, Myung Hee; Jeong, Kyoung Jin; Hong, Chein Soo; Yong, Tai Soon.

In: International Archives of Allergy and Immunology, Vol. 145, No. 1, 01.12.2007, p. 1-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sequence polymorphisms of major German cockroach allergens Bla g 1, Bla g 2, Bla g 4, and Bla g 5

AU - Jeong, Kyoung Yong

AU - Lee, Haeseok

AU - Shin, Kwang Hyun

AU - Yi, Myung Hee

AU - Jeong, Kyoung Jin

AU - Hong, Chein Soo

AU - Yong, Tai Soon

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Background: The allergenicity of allergens could be influenced by amino acid substitutions in B- or T-cell epitope regions. The German cockroach is known to produce potent allergens inducing strong IgE-mediated allergic reactions. This study was performed to investigate sequence variations in major allergens of the German cockroach. Methods: Reverse transcriptase PCR was used to amplify the cDNA sequences encoding major allergens of the German cockroach (Bla g 1, Bla g 2, Bla g 4, and Bla g 5). Results: The deduced amino acid sequences revealed 38 Bla g 1 variants with 1-7 amino acid substitutions (98.6-99.8% identity), 28 Bla g 2 variants with 1-3 substitutions (99.1-99.7%), 27 Bla g 4 variants with 0-32 substitutions (82.4-100%), and 8 Bla g 5 variants with 1-2 substitutions (99.0-99.5%), respectively. Bla g 1 and Bla g 2 showed sporadic amino acid substitutions despite the divergence in their sequences. Bla g 4 exhibited frequent variations, with clusters of substitutions in residues 29-38, 52-80, and 132-155. Sequence variations in Bla g 4 imply the presence of multiple isoforms and isoallergens, which may in turn have various effects on the IgE-binding capacity and T-cell responsiveness. Only 8 variants were found in Bla g 5, with infrequent amino acid changes of one or two residues. Conclusions: Analyses of T-cell and IgE-binding epitope regions would clarify the effect of sequence polymorphisms on allergenicity, which in turn will aid in the design of allergen formulations for diagnosis and immunotherapy for cockroach allergies.

AB - Background: The allergenicity of allergens could be influenced by amino acid substitutions in B- or T-cell epitope regions. The German cockroach is known to produce potent allergens inducing strong IgE-mediated allergic reactions. This study was performed to investigate sequence variations in major allergens of the German cockroach. Methods: Reverse transcriptase PCR was used to amplify the cDNA sequences encoding major allergens of the German cockroach (Bla g 1, Bla g 2, Bla g 4, and Bla g 5). Results: The deduced amino acid sequences revealed 38 Bla g 1 variants with 1-7 amino acid substitutions (98.6-99.8% identity), 28 Bla g 2 variants with 1-3 substitutions (99.1-99.7%), 27 Bla g 4 variants with 0-32 substitutions (82.4-100%), and 8 Bla g 5 variants with 1-2 substitutions (99.0-99.5%), respectively. Bla g 1 and Bla g 2 showed sporadic amino acid substitutions despite the divergence in their sequences. Bla g 4 exhibited frequent variations, with clusters of substitutions in residues 29-38, 52-80, and 132-155. Sequence variations in Bla g 4 imply the presence of multiple isoforms and isoallergens, which may in turn have various effects on the IgE-binding capacity and T-cell responsiveness. Only 8 variants were found in Bla g 5, with infrequent amino acid changes of one or two residues. Conclusions: Analyses of T-cell and IgE-binding epitope regions would clarify the effect of sequence polymorphisms on allergenicity, which in turn will aid in the design of allergen formulations for diagnosis and immunotherapy for cockroach allergies.

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