Sequentially addressable twodimensional microwell array for highthroughput single cell-based assay

Young Ji Kim, Hyung Kew Lee, Jaehoon Chung, Il Joo Cho, Euisik Yoon

Research output: Contribution to conferencePaper

Abstract

This paper proposes a new microfluidic array which can sequentially load different types of reagents into a two-dimensional microwell array for high-throughput single cell assay. Proposed array is composed of more than 200 chambers and each sub-array can be selected by microfluidic logics for row and column selection. By applying pneumatic pressure and injecting a different set of reagents, we could load different reagents into the two-dimensional array. In order to verify the sequential addressing scheme, we fabricated and tested a 3x3 device for 9 different reagent loading demonstrated by various colors in food dye.

Original languageEnglish
Pages483-485
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

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Microfluidics
Assays
Pneumatics
Dyes
Throughput
Color
Coloring Agents

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kim, Y. J., Lee, H. K., Chung, J., Cho, I. J., & Yoon, E. (2008). Sequentially addressable twodimensional microwell array for highthroughput single cell-based assay. 483-485. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.
Kim, Young Ji ; Lee, Hyung Kew ; Chung, Jaehoon ; Cho, Il Joo ; Yoon, Euisik. / Sequentially addressable twodimensional microwell array for highthroughput single cell-based assay. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.3 p.
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Kim, YJ, Lee, HK, Chung, J, Cho, IJ & Yoon, E 2008, 'Sequentially addressable twodimensional microwell array for highthroughput single cell-based assay' Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 08/10/12 - 08/10/16, pp. 483-485.

Sequentially addressable twodimensional microwell array for highthroughput single cell-based assay. / Kim, Young Ji; Lee, Hyung Kew; Chung, Jaehoon; Cho, Il Joo; Yoon, Euisik.

2008. 483-485 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conferencePaper

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Kim YJ, Lee HK, Chung J, Cho IJ, Yoon E. Sequentially addressable twodimensional microwell array for highthroughput single cell-based assay. 2008. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.