Serially Ordered Magnetization of Nanoclusters via Control of Various Transition Metal Dopants for the Multifractionation of Cells in Microfluidic Magnetophoresis Devices

Byunghoon Kang, Bumjoon Cha, Bongsoo Kim, Seungmin Han, Moo Kwang Shin, Eunji Jang, Hyun Ouk Kim, Seo Ryung Bae, Unyong Jeong, Il Moon, Hye Yeong Son, Yong Min Huh, Seungjoo Haam

Research output: Contribution to journalArticle

Abstract

A novel method (i.e., continuous magnetic cell separation in a microfluidic channel) is demonstrated to be capable of inducing multifractionation of mixed cell suspensions into multiple outlet fractions. Here, multicomponent cell separation is performed with three different distinguishable magnetic nanoclusters (MnFe2O4, Fe3O4, and CoFe2O4), which are tagged on A431 cells. Because of their mass magnetizations, which can be ideally altered by doping with magnetic atom compositions (Mn, Fe, and Co), the trajectories of cells with each magnetic nanocluster in a flow are shown to be distinct when dragged under the same external magnetic field; the rest of the magnetic characteristics of the nanoclusters are identically fixed. This proof of concept study, which utilizes the magnetization-controlled nanoclusters (NCs), suggests that precise and effective multifractionation is achievable with high-throughput and systematic accuracy for dynamic cell separation.

Original languageEnglish
Pages (from-to)1078-1082
Number of pages5
JournalAnalytical Chemistry
Volume88
Issue number2
DOIs
Publication statusPublished - 2016 Jan 19

Fingerprint

Nanoclusters
Microfluidics
Transition metals
Magnetization
Doping (additives)
Suspensions
Trajectories
Throughput
Magnetic fields
Atoms
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Kang, Byunghoon ; Cha, Bumjoon ; Kim, Bongsoo ; Han, Seungmin ; Shin, Moo Kwang ; Jang, Eunji ; Kim, Hyun Ouk ; Bae, Seo Ryung ; Jeong, Unyong ; Moon, Il ; Son, Hye Yeong ; Huh, Yong Min ; Haam, Seungjoo. / Serially Ordered Magnetization of Nanoclusters via Control of Various Transition Metal Dopants for the Multifractionation of Cells in Microfluidic Magnetophoresis Devices. In: Analytical Chemistry. 2016 ; Vol. 88, No. 2. pp. 1078-1082.
@article{a7f043354dd54e2087415882036183c8,
title = "Serially Ordered Magnetization of Nanoclusters via Control of Various Transition Metal Dopants for the Multifractionation of Cells in Microfluidic Magnetophoresis Devices",
abstract = "A novel method (i.e., continuous magnetic cell separation in a microfluidic channel) is demonstrated to be capable of inducing multifractionation of mixed cell suspensions into multiple outlet fractions. Here, multicomponent cell separation is performed with three different distinguishable magnetic nanoclusters (MnFe2O4, Fe3O4, and CoFe2O4), which are tagged on A431 cells. Because of their mass magnetizations, which can be ideally altered by doping with magnetic atom compositions (Mn, Fe, and Co), the trajectories of cells with each magnetic nanocluster in a flow are shown to be distinct when dragged under the same external magnetic field; the rest of the magnetic characteristics of the nanoclusters are identically fixed. This proof of concept study, which utilizes the magnetization-controlled nanoclusters (NCs), suggests that precise and effective multifractionation is achievable with high-throughput and systematic accuracy for dynamic cell separation.",
author = "Byunghoon Kang and Bumjoon Cha and Bongsoo Kim and Seungmin Han and Shin, {Moo Kwang} and Eunji Jang and Kim, {Hyun Ouk} and Bae, {Seo Ryung} and Unyong Jeong and Il Moon and Son, {Hye Yeong} and Huh, {Yong Min} and Seungjoo Haam",
year = "2016",
month = "1",
day = "19",
doi = "10.1021/acs.analchem.5b04111",
language = "English",
volume = "88",
pages = "1078--1082",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "2",

}

Serially Ordered Magnetization of Nanoclusters via Control of Various Transition Metal Dopants for the Multifractionation of Cells in Microfluidic Magnetophoresis Devices. / Kang, Byunghoon; Cha, Bumjoon; Kim, Bongsoo; Han, Seungmin; Shin, Moo Kwang; Jang, Eunji; Kim, Hyun Ouk; Bae, Seo Ryung; Jeong, Unyong; Moon, Il; Son, Hye Yeong; Huh, Yong Min; Haam, Seungjoo.

In: Analytical Chemistry, Vol. 88, No. 2, 19.01.2016, p. 1078-1082.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Serially Ordered Magnetization of Nanoclusters via Control of Various Transition Metal Dopants for the Multifractionation of Cells in Microfluidic Magnetophoresis Devices

AU - Kang, Byunghoon

AU - Cha, Bumjoon

AU - Kim, Bongsoo

AU - Han, Seungmin

AU - Shin, Moo Kwang

AU - Jang, Eunji

AU - Kim, Hyun Ouk

AU - Bae, Seo Ryung

AU - Jeong, Unyong

AU - Moon, Il

AU - Son, Hye Yeong

AU - Huh, Yong Min

AU - Haam, Seungjoo

PY - 2016/1/19

Y1 - 2016/1/19

N2 - A novel method (i.e., continuous magnetic cell separation in a microfluidic channel) is demonstrated to be capable of inducing multifractionation of mixed cell suspensions into multiple outlet fractions. Here, multicomponent cell separation is performed with three different distinguishable magnetic nanoclusters (MnFe2O4, Fe3O4, and CoFe2O4), which are tagged on A431 cells. Because of their mass magnetizations, which can be ideally altered by doping with magnetic atom compositions (Mn, Fe, and Co), the trajectories of cells with each magnetic nanocluster in a flow are shown to be distinct when dragged under the same external magnetic field; the rest of the magnetic characteristics of the nanoclusters are identically fixed. This proof of concept study, which utilizes the magnetization-controlled nanoclusters (NCs), suggests that precise and effective multifractionation is achievable with high-throughput and systematic accuracy for dynamic cell separation.

AB - A novel method (i.e., continuous magnetic cell separation in a microfluidic channel) is demonstrated to be capable of inducing multifractionation of mixed cell suspensions into multiple outlet fractions. Here, multicomponent cell separation is performed with three different distinguishable magnetic nanoclusters (MnFe2O4, Fe3O4, and CoFe2O4), which are tagged on A431 cells. Because of their mass magnetizations, which can be ideally altered by doping with magnetic atom compositions (Mn, Fe, and Co), the trajectories of cells with each magnetic nanocluster in a flow are shown to be distinct when dragged under the same external magnetic field; the rest of the magnetic characteristics of the nanoclusters are identically fixed. This proof of concept study, which utilizes the magnetization-controlled nanoclusters (NCs), suggests that precise and effective multifractionation is achievable with high-throughput and systematic accuracy for dynamic cell separation.

UR - http://www.scopus.com/inward/record.url?scp=84955471666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84955471666&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.5b04111

DO - 10.1021/acs.analchem.5b04111

M3 - Article

C2 - 26717968

AN - SCOPUS:84955471666

VL - 88

SP - 1078

EP - 1082

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 2

ER -