Serodiagnosis of human sparganosis by a monoclonal antibody-based competition ELISA.

I. S. Yeo, T. S. Yong, K. Im

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Competition ELISA test using sparganum specific monoclonal antibodies (Mab) was investigated to improve the diagnostic specificity of sparganosis. By cell fusion, one hybridoma clone secreting anti-sparganum specific Mab was selected (Sp-20), which reacted on bands of 32 kDa and 38 kDa. Sp-20 reacted on calcium corpuscles on IFA. By micro-ELISA, 16 of 17 sparganosis cases (95%) were found positive, but 1 of 18 clonorchiasis cases (5%), 4 of 16 cysticercosis cases (25%) and 2 of 16 normal controls (11%) showed false positive reactions. On the other hand, by competition ELISA using a sparganum specific Mab (Sp-20), 16 out of 17 (95%) of sparganosis cases were found positive, but 2 of 18 clonorchiasis cases (10%), 2 of 16 cysticercosis cases (12%), 3 of 16 paragonimiasis cases (18%) and 1 of 16 normal controls (6%) showed false positive reactions.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalYonsei medical journal
Volume35
Issue number1
DOIs
Publication statusPublished - 1994 Mar

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this