Serodiagnostic potential of Mycobacterium avium MAV2054 and MAV5183 proteins

A. Rum Shin, Kil Soo Lee, Kang In Lee, Tae Sun Shim, Won Jung Koh, Haet Sal Jeon, Yeo Jin Son, SungJae Shin, Hwa Jung Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Mycobacterium avium-M. intracellulare complex (MAC) causes a pulmonary disease (PD) similar to tuberculosis (TB). Diagnosis of MAC-PD is complicated and time-consuming. In this study, the serodiagnostic potential of the newly identified MAV2054 and MAV5183 proteins was evaluated in subjects with MAC-PD, pulmonary TB, or latent TB and in noninfected healthy controls (HC), together with HspX and the 38-kDa antigen, well-known serodiagnostic M. tuberculosis antigens. All four antigens evoked significantly higher IgG responses in MAC-PD and active TB than in latent TB and HC subjects. Among the antigens, MAV2054 elicited the highest antibody responses in pulmonary TB and MAC-PD patients. IgG titers against MAV2054 and MAV5183 were significantly higher in MAC-PD than in pulmonary TB subjects. In addition, the levels of IgG against all antigens in the M. intracellulare and fibrocavitary forms were higher than those in the M. avium and nodular bronchiectatic forms, respectively. Based on sensitivity and receiver operator characteristic curve analysis, the best candidates for detection of MAC-PD and pulmonary TB were MAV2054 and the 38-kDa antigen, respectively. In total, 76.0% of MAC-PD and 65.0% of active TB patients were reactive to at least two antigens. In contrast, only 2.8% of HC subjects were reactive with two or more antigens. Our findings suggest that an enzyme-linked immunosorbent assay (ELISA) using the four antigens would be valuable for screening for mycobacterial lung disease, including MAC-PD and pulmonary TB, although it does not provide good discrimination of the disease-causing pathogens.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalClinical and Vaccine Immunology
Volume20
Issue number2
DOIs
Publication statusPublished - 2013 Feb 1

Fingerprint

Mycobacterium avium
Pulmonary diseases
Mycobacterium avium Complex
Lung Diseases
Antigens
Pulmonary Tuberculosis
Proteins
Tuberculosis
Latent Tuberculosis
Immunoglobulin G
Healthy Volunteers
Immunosorbents
Pathogens
Antibody Formation
Assays
Screening
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Shin, A. R., Lee, K. S., Lee, K. I., Shim, T. S., Koh, W. J., Jeon, H. S., ... Kim, H. J. (2013). Serodiagnostic potential of Mycobacterium avium MAV2054 and MAV5183 proteins. Clinical and Vaccine Immunology, 20(2), 295-301. https://doi.org/10.1128/CVI.00649-12
Shin, A. Rum ; Lee, Kil Soo ; Lee, Kang In ; Shim, Tae Sun ; Koh, Won Jung ; Jeon, Haet Sal ; Son, Yeo Jin ; Shin, SungJae ; Kim, Hwa Jung. / Serodiagnostic potential of Mycobacterium avium MAV2054 and MAV5183 proteins. In: Clinical and Vaccine Immunology. 2013 ; Vol. 20, No. 2. pp. 295-301.
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Shin, AR, Lee, KS, Lee, KI, Shim, TS, Koh, WJ, Jeon, HS, Son, YJ, Shin, S & Kim, HJ 2013, 'Serodiagnostic potential of Mycobacterium avium MAV2054 and MAV5183 proteins', Clinical and Vaccine Immunology, vol. 20, no. 2, pp. 295-301. https://doi.org/10.1128/CVI.00649-12

Serodiagnostic potential of Mycobacterium avium MAV2054 and MAV5183 proteins. / Shin, A. Rum; Lee, Kil Soo; Lee, Kang In; Shim, Tae Sun; Koh, Won Jung; Jeon, Haet Sal; Son, Yeo Jin; Shin, SungJae; Kim, Hwa Jung.

In: Clinical and Vaccine Immunology, Vol. 20, No. 2, 01.02.2013, p. 295-301.

Research output: Contribution to journalArticle

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T1 - Serodiagnostic potential of Mycobacterium avium MAV2054 and MAV5183 proteins

AU - Shin, A. Rum

AU - Lee, Kil Soo

AU - Lee, Kang In

AU - Shim, Tae Sun

AU - Koh, Won Jung

AU - Jeon, Haet Sal

AU - Son, Yeo Jin

AU - Shin, SungJae

AU - Kim, Hwa Jung

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N2 - The Mycobacterium avium-M. intracellulare complex (MAC) causes a pulmonary disease (PD) similar to tuberculosis (TB). Diagnosis of MAC-PD is complicated and time-consuming. In this study, the serodiagnostic potential of the newly identified MAV2054 and MAV5183 proteins was evaluated in subjects with MAC-PD, pulmonary TB, or latent TB and in noninfected healthy controls (HC), together with HspX and the 38-kDa antigen, well-known serodiagnostic M. tuberculosis antigens. All four antigens evoked significantly higher IgG responses in MAC-PD and active TB than in latent TB and HC subjects. Among the antigens, MAV2054 elicited the highest antibody responses in pulmonary TB and MAC-PD patients. IgG titers against MAV2054 and MAV5183 were significantly higher in MAC-PD than in pulmonary TB subjects. In addition, the levels of IgG against all antigens in the M. intracellulare and fibrocavitary forms were higher than those in the M. avium and nodular bronchiectatic forms, respectively. Based on sensitivity and receiver operator characteristic curve analysis, the best candidates for detection of MAC-PD and pulmonary TB were MAV2054 and the 38-kDa antigen, respectively. In total, 76.0% of MAC-PD and 65.0% of active TB patients were reactive to at least two antigens. In contrast, only 2.8% of HC subjects were reactive with two or more antigens. Our findings suggest that an enzyme-linked immunosorbent assay (ELISA) using the four antigens would be valuable for screening for mycobacterial lung disease, including MAC-PD and pulmonary TB, although it does not provide good discrimination of the disease-causing pathogens.

AB - The Mycobacterium avium-M. intracellulare complex (MAC) causes a pulmonary disease (PD) similar to tuberculosis (TB). Diagnosis of MAC-PD is complicated and time-consuming. In this study, the serodiagnostic potential of the newly identified MAV2054 and MAV5183 proteins was evaluated in subjects with MAC-PD, pulmonary TB, or latent TB and in noninfected healthy controls (HC), together with HspX and the 38-kDa antigen, well-known serodiagnostic M. tuberculosis antigens. All four antigens evoked significantly higher IgG responses in MAC-PD and active TB than in latent TB and HC subjects. Among the antigens, MAV2054 elicited the highest antibody responses in pulmonary TB and MAC-PD patients. IgG titers against MAV2054 and MAV5183 were significantly higher in MAC-PD than in pulmonary TB subjects. In addition, the levels of IgG against all antigens in the M. intracellulare and fibrocavitary forms were higher than those in the M. avium and nodular bronchiectatic forms, respectively. Based on sensitivity and receiver operator characteristic curve analysis, the best candidates for detection of MAC-PD and pulmonary TB were MAV2054 and the 38-kDa antigen, respectively. In total, 76.0% of MAC-PD and 65.0% of active TB patients were reactive to at least two antigens. In contrast, only 2.8% of HC subjects were reactive with two or more antigens. Our findings suggest that an enzyme-linked immunosorbent assay (ELISA) using the four antigens would be valuable for screening for mycobacterial lung disease, including MAC-PD and pulmonary TB, although it does not provide good discrimination of the disease-causing pathogens.

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