Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy

Sangnae Cho, D. L. Yanagihara, S. W. Hunter, R. H. Gelber, P. J. Brennan

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

The serological activities of the specific phenolic glycolipid I from Mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-M. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. High anti-phenolic glycolipid I immunoglobulin M antibodies were found in 23 of 24 (96%) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tuberculoid leprosy patients (62%). Sera from patients with tuberculosis of atypical mycobacterial infections were devoid of anti-phenolic glycolipid I activity. The structurally related phenolic glycolipids from Mycobacterium kansasii and Mycobacterium bovis and the aglycone segments of the M. leprae product showed no significant activity. Thus, the trisaccharide determinant of phenolic glycolipid I is specific in its structure, serological activity, and, to a lesser extent, the antibody class it evokes.

Original languageEnglish
Pages (from-to)1077-1083
Number of pages7
JournalInfection and Immunity
Volume41
Issue number3
Publication statusPublished - 1983 Oct 27

Fingerprint

Glycolipids
Serologic Tests
Leprosy
Mycobacterium leprae
Mycobacterium kansasii
Tuberculoid Leprosy
Lepromatous Leprosy
Trisaccharides
Immunoglobulin Isotypes
Mycobacterium
Mycobacterium bovis
Serum
Immunoglobulin M
Immune Sera
Tuberculosis
Enzyme-Linked Immunosorbent Assay
Mycobacterium leprae phenolic glycolipid I
Rabbits
Drug Therapy
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Cho, S., Yanagihara, D. L., Hunter, S. W., Gelber, R. H., & Brennan, P. J. (1983). Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy. Infection and Immunity, 41(3), 1077-1083.
Cho, Sangnae ; Yanagihara, D. L. ; Hunter, S. W. ; Gelber, R. H. ; Brennan, P. J. / Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy. In: Infection and Immunity. 1983 ; Vol. 41, No. 3. pp. 1077-1083.
@article{d8e099966ff34324ae15cf8ad960b06b,
title = "Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy",
abstract = "The serological activities of the specific phenolic glycolipid I from Mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-M. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. High anti-phenolic glycolipid I immunoglobulin M antibodies were found in 23 of 24 (96{\%}) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tuberculoid leprosy patients (62{\%}). Sera from patients with tuberculosis of atypical mycobacterial infections were devoid of anti-phenolic glycolipid I activity. The structurally related phenolic glycolipids from Mycobacterium kansasii and Mycobacterium bovis and the aglycone segments of the M. leprae product showed no significant activity. Thus, the trisaccharide determinant of phenolic glycolipid I is specific in its structure, serological activity, and, to a lesser extent, the antibody class it evokes.",
author = "Sangnae Cho and Yanagihara, {D. L.} and Hunter, {S. W.} and Gelber, {R. H.} and Brennan, {P. J.}",
year = "1983",
month = "10",
day = "27",
language = "English",
volume = "41",
pages = "1077--1083",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

Cho, S, Yanagihara, DL, Hunter, SW, Gelber, RH & Brennan, PJ 1983, 'Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy', Infection and Immunity, vol. 41, no. 3, pp. 1077-1083.

Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy. / Cho, Sangnae; Yanagihara, D. L.; Hunter, S. W.; Gelber, R. H.; Brennan, P. J.

In: Infection and Immunity, Vol. 41, No. 3, 27.10.1983, p. 1077-1083.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy

AU - Cho, Sangnae

AU - Yanagihara, D. L.

AU - Hunter, S. W.

AU - Gelber, R. H.

AU - Brennan, P. J.

PY - 1983/10/27

Y1 - 1983/10/27

N2 - The serological activities of the specific phenolic glycolipid I from Mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-M. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. High anti-phenolic glycolipid I immunoglobulin M antibodies were found in 23 of 24 (96%) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tuberculoid leprosy patients (62%). Sera from patients with tuberculosis of atypical mycobacterial infections were devoid of anti-phenolic glycolipid I activity. The structurally related phenolic glycolipids from Mycobacterium kansasii and Mycobacterium bovis and the aglycone segments of the M. leprae product showed no significant activity. Thus, the trisaccharide determinant of phenolic glycolipid I is specific in its structure, serological activity, and, to a lesser extent, the antibody class it evokes.

AB - The serological activities of the specific phenolic glycolipid I from Mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-M. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. High anti-phenolic glycolipid I immunoglobulin M antibodies were found in 23 of 24 (96%) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tuberculoid leprosy patients (62%). Sera from patients with tuberculosis of atypical mycobacterial infections were devoid of anti-phenolic glycolipid I activity. The structurally related phenolic glycolipids from Mycobacterium kansasii and Mycobacterium bovis and the aglycone segments of the M. leprae product showed no significant activity. Thus, the trisaccharide determinant of phenolic glycolipid I is specific in its structure, serological activity, and, to a lesser extent, the antibody class it evokes.

UR - http://www.scopus.com/inward/record.url?scp=0020599815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020599815&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 1077

EP - 1083

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -