Biosensors based on surface-enhanced Raman scattering (SERS) represent an efficient tool that can quickly detect even very low concentrations of the target molecule. Recent SERS-based biosensing has been moving toward a multiplex detection platform to analyze samples containing a variety of molecules, such as blood and urine. In this study, shape-coded suspension arrays have been developed for SERS-based multiplex biosensing. The suspension microarrays were fabricated using different shapes of poly(ethylene glycol) (PEG)-based hydrogel microparticles, which were prepared by a simple photopatterning process. The resultant hydrogel particles were coated with silver nanoparticles by a silver mirror reaction and then divided into two groups depending on the shape. As a proof of concept, square-shaped particles were coated with 4-mercaptophenylboronic acid by self-assembly to detect glucose, while circular particles were functionalized with anti-human serum albumin (HSA) to detect HSA. The assay was performed in the concentration range of 1 pg/mL to 1 μg/mL for both glucose and HSA without crosstalk. Target selectivity was also confirmed from Raman mapping, which shows that each group of particles reveals a different pattern. Moreover, we verified the potential of our method in multiplex immunoassays by quantifying two different antigens using SERS nanotags. The performance of the developed SERS-based multiplex immunoassay platform was demonstrated by the detection of a very low concentration of biomolecules with excellent selectivity.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning and the Korea government (MSIT) (2017M3D1A1039289 and 2017M3A7B4041798).
This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT , and Future Planning and the Korea government (MSIT) ( 2017M3D1A1039289 and 2017M3A7B4041798 ).
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry