Background: Exosomal long noncoding RNAs (lncRNAs) are known as ideal diagnostic biomarkers of various diseases. However, there are no reports on the use of serum exosomal lncRNAs as diagnostic biomarkers for atrial fibrillation (AF). Objective: The purpose of this study was to explore serum exosomal lncRNAs as a useful tool for diagnosing AF. Methods: Serum exosomes from patients with persistent AF and controls were isolated using a polymer-based exosome precipitation kit. We conducted a multiphase process including screening and 2 independent validation phases. In the screening phase, serum exosomal lncRNA expression profiles were examined using RNA sequencing analysis. In 2 validation phases, we evaluated the expression levels of candidate exosomal lncRNAs using quantitative reverse transcription polymerase chain reaction. Finally, we performed different statistical and functional analyses. Results: After the screening phase, we identified 26 differentially expressed lncRNAs (ie, 15 upregulated and 11 downregulated lncRNAs with a |fold change| ≥2 and P <.05) in serum exosomes from patients with persistent AF compared with controls. We then screened out 6 exosomal lncRNAs as biomarker candidates following parameters: read length ≥200 nucleotides; exon number ≥2; and coding potential score <0.1. In 2 validation phases, exosomal lncRNAs LOC105377989 and LOC107986997 were consistently upregulated in the serum of patients with persistent AF compared with controls (P <.0001). Moreover, both exosomal lncRNAs exhibited significant diagnostic validity for AF. Notably, exosomal lncRNA LOC107986997 was involved in AF-related pathophysiological mechanisms. Conclusion: Serum-derived exosomal lncRNA LOC107986997 could serve as a potential biomarker for AF diagnosis.
Bibliographical noteFunding Information:
Funding Sources: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2021R1I1A1A01052197 ); and the Korean Fund for Regenerative Medicine funded by the Ministry of Science and ICT and Ministry of Health and Welfare ( KFRM21B0604L1-01 ).
Funding Sources: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01052197); and the Korean Fund for Regenerative Medicine funded by the Ministry of Science and ICT and Ministry of Health and Welfare (KFRM21B0604L1-01).We would like to thank Editage (www.editage.co.kr) for English language editing, Bioinformatics Collaboration Unit (BiCU) in the Department of Biomedical Systems Informatics, and Medical Illustration & Design, part of the Medical Research Support Services of Yonsei University College of Medicine, for all artistic support related to this work.
© 2022 Heart Rhythm Society
All Science Journal Classification (ASJC) codes
- Cardiology and Cardiovascular Medicine
- Physiology (medical)