SET domain-containing protein 5 is required for expression of primordial germ cell specification-associated genes in murine embryonic stem cells

Seung Eun Yu, Min Seong Kim, Su Hyung Park, Byong Chul Yoo, Kyung Hee Kim, Yeun Kyu Jang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Primordial germ cell (PGC) specification is one of the most fundamental processes in developmental biology. Because PGCs are a common source of both gametes, generation of PGCs from embryonic stem cells (ESCs) is a useful model for analysing the germ line lineage. Although several studies focused on the role of epigenetic regulation on PGC differentiation from ESCs in vitro have been published, germ line commitment remains poorly understood. Here, we show that SET domain-containing protein (Setd5), which has a previously unknown function, is essential for regulating germ cell-associated genes in murine ESCs (mESCs). Even though Setd5 knockdown with 3 distinct shRNAs did not affect expression of pluripotency genes or levels of global histone methylation, all 3 shRNAs significantly diminished the expression of early and late-stage PGC-associated genes. Furthermore, our immunoprecipitation assay showed that Setd5 can interact with Tbl1xr1 and Ctr9, which are components of 2 different transcriptional regulatory complexes, namely, NcoR1 corepressor complex and Paf1 complex, respectively, in mESCs. Taken together, our data suggest that Setd5 is required for maintaining PGC-associated genes and Setd5-associated protein complexes containing Tbl1xr1 and Ctr9, which in turn are likely involved in regulating germ cell–related genes in mESCs.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalCell Biochemistry and Function
Volume35
Issue number5
DOIs
Publication statusPublished - 2017 Jul 1

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Embryonic Stem Cells
Stem cells
Germ Cells
Genes
Cells
Specifications
Proteins
Co-Repressor Proteins
Methylation
Histones
Assays
Developmental Biology
Protein Domains
Immunoprecipitation
Epigenomics
Cell Differentiation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

Yu, Seung Eun ; Kim, Min Seong ; Park, Su Hyung ; Yoo, Byong Chul ; Kim, Kyung Hee ; Jang, Yeun Kyu. / SET domain-containing protein 5 is required for expression of primordial germ cell specification-associated genes in murine embryonic stem cells. In: Cell Biochemistry and Function. 2017 ; Vol. 35, No. 5. pp. 247-253.
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SET domain-containing protein 5 is required for expression of primordial germ cell specification-associated genes in murine embryonic stem cells. / Yu, Seung Eun; Kim, Min Seong; Park, Su Hyung; Yoo, Byong Chul; Kim, Kyung Hee; Jang, Yeun Kyu.

In: Cell Biochemistry and Function, Vol. 35, No. 5, 01.07.2017, p. 247-253.

Research output: Contribution to journalArticle

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