Shh signaling is essential for rugae morphogenesis in mice

Jong Min Lee, Seita Miyazawa, Jeong Oh Shin, Hyuk Jae Kwon, Dae Woon Kang, Byung Jai Choi, Jae Ho Lee, Shigeru Kondo, Sung Won Cho, Hansung Jung

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Palatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae.

Original languageEnglish
Pages (from-to)663-675
Number of pages13
JournalHistochemistry and cell biology
Volume136
Issue number6
DOIs
Publication statusPublished - 2011 Dec 1

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Morphogenesis
Hard Palate
Wnt Signaling Pathway
Palate
Anti-Idiotypic Antibodies
Embryonic Structures
Mothers
Cell Proliferation
Apoptosis
Gene Expression
Injections
Genes
Inhibition (Psychology)
Transfer (Psychology)

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Lee, J. M., Miyazawa, S., Shin, J. O., Kwon, H. J., Kang, D. W., Choi, B. J., ... Jung, H. (2011). Shh signaling is essential for rugae morphogenesis in mice. Histochemistry and cell biology, 136(6), 663-675. https://doi.org/10.1007/s00418-011-0870-7
Lee, Jong Min ; Miyazawa, Seita ; Shin, Jeong Oh ; Kwon, Hyuk Jae ; Kang, Dae Woon ; Choi, Byung Jai ; Lee, Jae Ho ; Kondo, Shigeru ; Cho, Sung Won ; Jung, Hansung. / Shh signaling is essential for rugae morphogenesis in mice. In: Histochemistry and cell biology. 2011 ; Vol. 136, No. 6. pp. 663-675.
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abstract = "Palatal ridges, or rugae palatinae, are corrugated structures observed in the hard palate region. They are found in most mammalian species, but their number and arrangement are species-specific. Nine palatal rugae are found in the mouse secondary palate. Previous studies have shown that epithelial Shh signaling in the palatal ridge plays an important role during rugae development. Moreover, Wnt family members, including LEF1, play a functional role in orofacial morphogenesis. To explore the function of Shh during rugae development, we utilized the maternal transfer of 5E1 (anti-Shh antibody) to mouse embryos. 5E1 induced abnormal rugae patterning characterized by a spotted shape of palatal ridge rather than a stripe. The expression patterns of Shh and Shh-related genes, Sostdc1, Lef1 and Ptch1, were disrupted following 5E1 injection. Moreover, rugae-specific cell proliferation and inter-rugae-specific apoptosis were affected by inhibition of Shh signaling. We hypothesize that the altered gene expression patterns and the change in molecular events caused by the inhibition of Shh signaling may have induced abnormal rugae patterning. Furthermore, we propose a reaction-diffusion model generated by Wnt, Shh and Sostdc1 signaling. In this study, we show that Sostdc1, a secreted inhibitor of the Wnt pathway, is a downstream target of Shh and hypothesize that the interaction of Wnt, Shh and Sostdc1 is a pivotal mechanism controlling the spatial patterning of palatal rugae.",
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Lee, JM, Miyazawa, S, Shin, JO, Kwon, HJ, Kang, DW, Choi, BJ, Lee, JH, Kondo, S, Cho, SW & Jung, H 2011, 'Shh signaling is essential for rugae morphogenesis in mice', Histochemistry and cell biology, vol. 136, no. 6, pp. 663-675. https://doi.org/10.1007/s00418-011-0870-7

Shh signaling is essential for rugae morphogenesis in mice. / Lee, Jong Min; Miyazawa, Seita; Shin, Jeong Oh; Kwon, Hyuk Jae; Kang, Dae Woon; Choi, Byung Jai; Lee, Jae Ho; Kondo, Shigeru; Cho, Sung Won; Jung, Hansung.

In: Histochemistry and cell biology, Vol. 136, No. 6, 01.12.2011, p. 663-675.

Research output: Contribution to journalArticle

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AU - Lee, Jong Min

AU - Miyazawa, Seita

AU - Shin, Jeong Oh

AU - Kwon, Hyuk Jae

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AU - Choi, Byung Jai

AU - Lee, Jae Ho

AU - Kondo, Shigeru

AU - Cho, Sung Won

AU - Jung, Hansung

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Lee JM, Miyazawa S, Shin JO, Kwon HJ, Kang DW, Choi BJ et al. Shh signaling is essential for rugae morphogenesis in mice. Histochemistry and cell biology. 2011 Dec 1;136(6):663-675. https://doi.org/10.1007/s00418-011-0870-7