The aim of this study was to evaluate the feasibility of biological hydrogen production (BHP) in a dynamic membrane bioreactor. Hydraulic retention time (HRT) was varied from 1 to 12 hours, and the microbial communities were analyzed. The major hydrogen-producing bacterium was Clostridium chromiireducens, which was enriched up to 82% of the total bacteria with a 2 hour HRT, while Enterobacter cloacae was also observed in the range of 0.2% to 34.4% of total bacteria. When the level of the substrate in the reactor was low (<0.5 g/L), BHP dropped dramatically, and Sporolactobacillus putidus increased to 8.8% of the total bacteria. Differences in substrate affinity between S. putidus and C. chromiireducens likely promoted the dominance of S. putidus, which is more favorable for growth and metabolism at low substrate concentrations, and caused changes in the microbial community.
All Science Journal Classification (ASJC) codes
- Renewable Energy, Sustainability and the Environment
- Nuclear Energy and Engineering
- Fuel Technology
- Energy Engineering and Power Technology