Short communication: Prospective comparison of qualitative versus quantitative polymerase chain reaction for monitoring virologic treatment failure in HIV-infected patients

Su Jin Jeong, Min Hyung Kim, Je Eun Song, Jin Young Ahn, Sun Bean Kim, Hea Won Ann, Jae Kyung Kim, Heun Choi, Nam Su Ku, Sang Hoon Han, June Myung Kim, Davey M. Smith, Hyon Suk Kim, Jun Yong Choi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Less costly but still accurate methods for monitoring HIV treatment response are needed. We prospectively evaluated if a qualitative polymerase chain reaction (PCR) amplification assay for virologic monitoring could maintain accuracy while reducing costs in Seoul, South Korea. We conducted the first prospective study comparing a qualitative PCR amplification of HIV-1 reverse transcriptase (RT) versus a commercial real time PCR assay (i.e., viral load) for virologic monitoring of 150 patients receiving antiretroviral therapy (ART) between November 2011 and August 2012 at an urban hospital in Seoul, South Korea. A total of 215 blood plasma samples from 150 patients receiving ART for more than 6 months were evaluated. Using the individual viral load assay, 12 of 215 (5.6%) plasma samples had more than 500 HIV RNA copies/ml. The qualitative PCR amplification assay detected individual samples with ≥500 HIV RNA copies/ml with 100% sensitivity. The specificities of the qualitative PCR amplification of the HIV-1 RT assay were 94.1%, 93.6%, and 93.2% compared to the real time PCR at 500, 1,000, and 5,000 threshold of HIV RNA copies/ml, respectively, and $24,940 USD would have been saved for 150 patients during 10 months. The qualitative PCR amplification of the HIV-1 RT assay might be a useful approach to effectively monitor patients receiving ART and save resources.

Original languageEnglish
Pages (from-to)827-829
Number of pages3
JournalAIDS Research and Human Retroviruses
Volume30
Issue number8
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology
  • Infectious Diseases

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