'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules

Hwangbeom Kim, Hyojun Han, Jinwoo Ahn, Joongoo Lee, Namjin Cho, Hoon Jang, Hyoki Kim, Sunghoon Kwon, Duhee Bang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.

Original languageEnglish
Pages (from-to)e140
JournalNucleic acids research
Volume40
Issue number18
DOIs
Publication statusPublished - 2012 Oct

All Science Journal Classification (ASJC) codes

  • Genetics

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