Shotgun lipidomics for candidate biomarkers of urinary phospholipids in prostate cancer

Hye Kyeong Min, Sangsoo Lim, Bong Chul Chung, Myeong Hee Moon

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Qualitative and quantitative profiling of six different categories of urinary phospholipids (PLs) from patients with prostate cancer was performed to develop an analytical method for the discovery of candidate biomarkers by shotgun lipidomics method. Using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry, we identified the molecular structures of a total of 70 PL molecules (21 phosphatidylcholines (PCs), 11 phosphatidylethanolamines (PEs), 17 phosphatidylserines (PSs), 11 phosphatidylinositols (PIs), seven phosphatidic acids, and three phosphatidylglycerols) from urine samples of healthy controls and prostate cancer patients by data-dependent collision-induced dissociation. Identified molecules were quantitatively examined by comparing the MS peak areas. From statistical analyses, one PC, one PE, six PSs, and two PIs among the PL species showed significant differences between controls and cancer patients (p∈<∈0.05, Student's t test), with concentration changes of more than threefold. Cluster analysis of both control and patient groups showed that 18:0/18:1-PS and 16:0/22:6-PS were 99% similar in upregulation and that the two PSs (18:1/18:0, 18:0/20:5) with two PIs (18:0/18:1 and 16:1/20:2) showed similar (>95%) downregulation. The total amount of each PL group was compared among prostate cancer patients according to the Gleason scale as larger or smaller than 6. It proposes that the current study can be utilized to sort out possible diagnostic biomarkers of prostate cancer.

Original languageEnglish
Pages (from-to)823-830
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume399
Issue number2
DOIs
Publication statusPublished - 2011 Jan 1

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Firearms
Biomarkers
Phospholipids
Prostatic Neoplasms
Phosphatidylethanolamines
Phosphatidylserines
Phosphatidylinositols
Phosphatidylcholines
Electrospray ionization
Phosphatidylglycerols
Phosphatidic Acids
Molecules
Electrospray Ionization Mass Spectrometry
Liquid chromatography
Tandem Mass Spectrometry
Molecular Structure
Liquid Chromatography
Molecular structure
Mass spectrometry
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Min, Hye Kyeong ; Lim, Sangsoo ; Chung, Bong Chul ; Moon, Myeong Hee. / Shotgun lipidomics for candidate biomarkers of urinary phospholipids in prostate cancer. In: Analytical and Bioanalytical Chemistry. 2011 ; Vol. 399, No. 2. pp. 823-830.
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Shotgun lipidomics for candidate biomarkers of urinary phospholipids in prostate cancer. / Min, Hye Kyeong; Lim, Sangsoo; Chung, Bong Chul; Moon, Myeong Hee.

In: Analytical and Bioanalytical Chemistry, Vol. 399, No. 2, 01.01.2011, p. 823-830.

Research output: Contribution to journalArticle

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