Abstract
This is a one-step POC biosensor that guarantees rapid assay times, low-cost analysis, simple handling, stability, and is easy to mass product. However, it has several drawbacks such as limited sample volume and relatively low sensitivity. To overcome these disadvantages, we have designed and fabricated the µ-PAD to confine fluid flow by creating a hydrophobic channel in the paper to improve the sensitivity. The channel pattern was drawn by a computer-aided design program and directly printed by a commercially available wax printer which generates the hydrophobic channel pattern. While maintaining a constant sample, absorption, and conjugation pad, the width of the detection pad was reduced from 5 mm to 2 mm at intervals of 1 mm. The intensity of bands from the single- and double-orifice patterns increased identically compared with the device with no orifice. The relative intensity of the signal bands increased from 141 to 158. Also, numerical simulation was performed to validate the experimental result by using lattice Boltzmann method. We observed that the sensitivity was enhanced at a specific detection pad width (3 mm). Therefore, our simple structural change of the channel led to improve the colorimetric intensity. Cortisol, which is a known stress biomarker, was used to validation the device, an enhanced signal was obtained indicating that our device can be used for the detection psychological stress in humans.
| Original language | English |
|---|---|
| Pages (from-to) | 116-123 |
| Number of pages | 8 |
| Journal | Biochip Journal |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2015 Jun 27 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Electrical and Electronic Engineering
Cite this
}
Signal amplification in a microfluidic paper-based analytical device (µ-PAD) by confinement of the fluidic flow. / Choi, Seoyeon; Lee, Jung Hyun; Kwak, Bong Seop; Kim, Young Woo; Lee, Joon Sang; Choi, Jong Soon; Jung, Hyo Il.
In: Biochip Journal, Vol. 9, No. 2, 27.06.2015, p. 116-123.Research output: Contribution to journal › Article
TY - JOUR
T1 - Signal amplification in a microfluidic paper-based analytical device (µ-PAD) by confinement of the fluidic flow
AU - Choi, Seoyeon
AU - Lee, Jung Hyun
AU - Kwak, Bong Seop
AU - Kim, Young Woo
AU - Lee, Joon Sang
AU - Choi, Jong Soon
AU - Jung, Hyo Il
PY - 2015/6/27
Y1 - 2015/6/27
N2 - This is a one-step POC biosensor that guarantees rapid assay times, low-cost analysis, simple handling, stability, and is easy to mass product. However, it has several drawbacks such as limited sample volume and relatively low sensitivity. To overcome these disadvantages, we have designed and fabricated the µ-PAD to confine fluid flow by creating a hydrophobic channel in the paper to improve the sensitivity. The channel pattern was drawn by a computer-aided design program and directly printed by a commercially available wax printer which generates the hydrophobic channel pattern. While maintaining a constant sample, absorption, and conjugation pad, the width of the detection pad was reduced from 5 mm to 2 mm at intervals of 1 mm. The intensity of bands from the single- and double-orifice patterns increased identically compared with the device with no orifice. The relative intensity of the signal bands increased from 141 to 158. Also, numerical simulation was performed to validate the experimental result by using lattice Boltzmann method. We observed that the sensitivity was enhanced at a specific detection pad width (3 mm). Therefore, our simple structural change of the channel led to improve the colorimetric intensity. Cortisol, which is a known stress biomarker, was used to validation the device, an enhanced signal was obtained indicating that our device can be used for the detection psychological stress in humans.
AB - This is a one-step POC biosensor that guarantees rapid assay times, low-cost analysis, simple handling, stability, and is easy to mass product. However, it has several drawbacks such as limited sample volume and relatively low sensitivity. To overcome these disadvantages, we have designed and fabricated the µ-PAD to confine fluid flow by creating a hydrophobic channel in the paper to improve the sensitivity. The channel pattern was drawn by a computer-aided design program and directly printed by a commercially available wax printer which generates the hydrophobic channel pattern. While maintaining a constant sample, absorption, and conjugation pad, the width of the detection pad was reduced from 5 mm to 2 mm at intervals of 1 mm. The intensity of bands from the single- and double-orifice patterns increased identically compared with the device with no orifice. The relative intensity of the signal bands increased from 141 to 158. Also, numerical simulation was performed to validate the experimental result by using lattice Boltzmann method. We observed that the sensitivity was enhanced at a specific detection pad width (3 mm). Therefore, our simple structural change of the channel led to improve the colorimetric intensity. Cortisol, which is a known stress biomarker, was used to validation the device, an enhanced signal was obtained indicating that our device can be used for the detection psychological stress in humans.
UR - http://www.scopus.com/inward/record.url?scp=84932605206&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84932605206&partnerID=8YFLogxK
U2 - 10.1007/s13206-015-9204-5
DO - 10.1007/s13206-015-9204-5
M3 - Article
AN - SCOPUS:84932605206
VL - 9
SP - 116
EP - 123
JO - Biochip Journal
JF - Biochip Journal
SN - 1976-0280
IS - 2
ER -