Signal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells

Jangwon Lee, Jeonghun Seo, Hye Young Kim, Jae Bock Chung, Kyung Hwan Kim

Research output: Contribution to journalArticle

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Abstract

The signaling pathways mediating cytokine production and apoptosis in pancreatic acinar cells have not been fully understood. We investigated the signal transduction of cerulein-induced cytokine IL-6 expression and apoptosis in pancreatic acinar AR42J cells. The wild-type cells and the cells transfected with mutant genes for signaling molecules (such as transcription factor NF-κB and AP-1) and ras, the upstream signal for mitogen-activated protein kinase (MAPK), were used. IL-6 level was determined in the medium by enzyme-linked immunosorbent assay (ELISA). Apoptosis was determined by viable cell counts and DNA fragmentation. Wild-type cells and transfected cells with control vector (pcDNA), IκB mutant gene (IκB mt), H-ras mutant gene (Ras N17), or c-jun dominant negative gene (TAM-67) were treated with cerulein for 24 hours. As a result, cerulein induced IL-6 expression time-dependently at 10 -8 M, and apoptosis dose-dependently at 24 h in the wild-type cells. Cerulein-induced IL-6 expression (at 10 -8 M) and apoptosis (at 10 -7 M) were inhibited in the transfected cells with mutant gene (IκB mt, Ras N17, TAM-67) as compared to pcDNA cells and the wild-type cells. In conclusion, cerulein induces cytokine expression and apoptotic cell death, which may be regulated by NF-κB, AP-1, and possibly MAPK in pancreatic acinar cells.

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalAnnals of the New York Academy of Sciences
Volume1010
DOIs
Publication statusPublished - 2003 Jan 1

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Ceruletide
Signal transduction
Acinar Cells
Signal Transduction
Genes
Apoptosis
Cytokines
Interleukin-6
Transcription Factor AP-1
Mitogen-Activated Protein Kinases
ras Genes
Immunosorbents
jun Genes
Cell death
Dominant Genes
Transduction
Cells
Assays
DNA Fragmentation
Transcription Factors

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Lee, Jangwon ; Seo, Jeonghun ; Kim, Hye Young ; Chung, Jae Bock ; Kim, Kyung Hwan. / Signal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells. In: Annals of the New York Academy of Sciences. 2003 ; Vol. 1010. pp. 104-108.
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Signal Transduction of Cerulein-Induced Cytokine Expression and Apoptosis in Pancreatic Acinar Cells. / Lee, Jangwon; Seo, Jeonghun; Kim, Hye Young; Chung, Jae Bock; Kim, Kyung Hwan.

In: Annals of the New York Academy of Sciences, Vol. 1010, 01.01.2003, p. 104-108.

Research output: Contribution to journalArticle

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