Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells

Ok Cho Soon, Hwan Kim Kyung, Joo Heon Yoon, Hye Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

17 Citations (Scopus)

Abstract

Integrin expression in cancer tissues demonstrates its possible contribution to tumor progression, invasion, and metastasis. Helicobacter pylori (H. pylori) infection is related to gastric cancer and gastric inflammation. H. pylori induced upregulation in expression of integrin in gastric epithelia cells. Reactive oxygen species (ROS) are considered as an important regulator in the pathogenesis of H. pylori-induced gastric ulceration and carcinogenesis. Integrin expression may be regulated by oxidant-sensitive transcription factors, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori in aKorean isolate (HP99) induces the expression of integrin α5 and integrin β1, and whether H. pylori-induced expression of integrin α5 and integrin β1 are inhibited in the cells transfected with mutant genes for Ras (ras N-17), c-Jun (TAM-67), and IκBα(MAD-3) or treated with DPI, an inhibitor of NADPH oxidase. As a result, H. pylori induced the expression of integrin α5 and integrin β1 in gastric adenocarcinoma (AGS) cells time-dependently. Treatment of DPI or transfection with mutant genes for Ras (ras N-17), c-jun (TAM67), and IκBα(MAD3) inhibited H. pylori-induced expression of integrin α5 and integrin β1 in AGS cells. In conclusion, H. pylori activates Ras, NF-κB, and AP-1 and thus induces the expression of integrin α5 and integrin β1 in gastric epithelial cells. Inhibition of ROS production by DPI suppressed the expression of integrin α5 and integrin β1 in gastric epithelial cells. The results suggest the possible involvement of NADPH oxidase for ROS production in H. pylori-infected gastric epithelial cells.

Original languageEnglish
Title of host publicationSignal Transduction Pathways, Part A
Subtitle of host publicationApoptotic and Extracellular Signalling
PublisherBlackwell Publishing Inc.
Pages298-304
Number of pages7
ISBN (Print)1573316458, 9781573316453
DOIs
Publication statusPublished - 2006 Jan 1

Publication series

NameAnnals of the New York Academy of Sciences
Volume1090
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Helicobacter pylori
Integrins
Stomach
Epithelial Cells
Reactive Oxygen Species
ras Genes
NADPH Oxidase
Transcription Factor AP-1
Cells
Stomach Neoplasms
Genes
Helicobacter Infections
Oxidants
Transfection
Tumors
Neoplasms
Carcinogenesis
Adenocarcinoma
Transcription Factors
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Soon, O. C., Kyung, H. K., Yoon, J. H., & Kim, H. Y. (2006). Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells. In Signal Transduction Pathways, Part A: Apoptotic and Extracellular Signalling (pp. 298-304). (Annals of the New York Academy of Sciences; Vol. 1090). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1378.032
Soon, Ok Cho ; Kyung, Hwan Kim ; Yoon, Joo Heon ; Kim, Hye Young. / Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells. Signal Transduction Pathways, Part A: Apoptotic and Extracellular Signalling. Blackwell Publishing Inc., 2006. pp. 298-304 (Annals of the New York Academy of Sciences).
@inproceedings{6401e43cdd7c449099751f2c8e72d8d7,
title = "Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells",
abstract = "Integrin expression in cancer tissues demonstrates its possible contribution to tumor progression, invasion, and metastasis. Helicobacter pylori (H. pylori) infection is related to gastric cancer and gastric inflammation. H. pylori induced upregulation in expression of integrin in gastric epithelia cells. Reactive oxygen species (ROS) are considered as an important regulator in the pathogenesis of H. pylori-induced gastric ulceration and carcinogenesis. Integrin expression may be regulated by oxidant-sensitive transcription factors, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori in aKorean isolate (HP99) induces the expression of integrin α5 and integrin β1, and whether H. pylori-induced expression of integrin α5 and integrin β1 are inhibited in the cells transfected with mutant genes for Ras (ras N-17), c-Jun (TAM-67), and IκBα(MAD-3) or treated with DPI, an inhibitor of NADPH oxidase. As a result, H. pylori induced the expression of integrin α5 and integrin β1 in gastric adenocarcinoma (AGS) cells time-dependently. Treatment of DPI or transfection with mutant genes for Ras (ras N-17), c-jun (TAM67), and IκBα(MAD3) inhibited H. pylori-induced expression of integrin α5 and integrin β1 in AGS cells. In conclusion, H. pylori activates Ras, NF-κB, and AP-1 and thus induces the expression of integrin α5 and integrin β1 in gastric epithelial cells. Inhibition of ROS production by DPI suppressed the expression of integrin α5 and integrin β1 in gastric epithelial cells. The results suggest the possible involvement of NADPH oxidase for ROS production in H. pylori-infected gastric epithelial cells.",
author = "Soon, {Ok Cho} and Kyung, {Hwan Kim} and Yoon, {Joo Heon} and Kim, {Hye Young}",
year = "2006",
month = "1",
day = "1",
doi = "10.1196/annals.1378.032",
language = "English",
isbn = "1573316458",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing Inc.",
pages = "298--304",
booktitle = "Signal Transduction Pathways, Part A",

}

Soon, OC, Kyung, HK, Yoon, JH & Kim, HY 2006, Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells. in Signal Transduction Pathways, Part A: Apoptotic and Extracellular Signalling. Annals of the New York Academy of Sciences, vol. 1090, Blackwell Publishing Inc., pp. 298-304. https://doi.org/10.1196/annals.1378.032

Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells. / Soon, Ok Cho; Kyung, Hwan Kim; Yoon, Joo Heon; Kim, Hye Young.

Signal Transduction Pathways, Part A: Apoptotic and Extracellular Signalling. Blackwell Publishing Inc., 2006. p. 298-304 (Annals of the New York Academy of Sciences; Vol. 1090).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells

AU - Soon, Ok Cho

AU - Kyung, Hwan Kim

AU - Yoon, Joo Heon

AU - Kim, Hye Young

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Integrin expression in cancer tissues demonstrates its possible contribution to tumor progression, invasion, and metastasis. Helicobacter pylori (H. pylori) infection is related to gastric cancer and gastric inflammation. H. pylori induced upregulation in expression of integrin in gastric epithelia cells. Reactive oxygen species (ROS) are considered as an important regulator in the pathogenesis of H. pylori-induced gastric ulceration and carcinogenesis. Integrin expression may be regulated by oxidant-sensitive transcription factors, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori in aKorean isolate (HP99) induces the expression of integrin α5 and integrin β1, and whether H. pylori-induced expression of integrin α5 and integrin β1 are inhibited in the cells transfected with mutant genes for Ras (ras N-17), c-Jun (TAM-67), and IκBα(MAD-3) or treated with DPI, an inhibitor of NADPH oxidase. As a result, H. pylori induced the expression of integrin α5 and integrin β1 in gastric adenocarcinoma (AGS) cells time-dependently. Treatment of DPI or transfection with mutant genes for Ras (ras N-17), c-jun (TAM67), and IκBα(MAD3) inhibited H. pylori-induced expression of integrin α5 and integrin β1 in AGS cells. In conclusion, H. pylori activates Ras, NF-κB, and AP-1 and thus induces the expression of integrin α5 and integrin β1 in gastric epithelial cells. Inhibition of ROS production by DPI suppressed the expression of integrin α5 and integrin β1 in gastric epithelial cells. The results suggest the possible involvement of NADPH oxidase for ROS production in H. pylori-infected gastric epithelial cells.

AB - Integrin expression in cancer tissues demonstrates its possible contribution to tumor progression, invasion, and metastasis. Helicobacter pylori (H. pylori) infection is related to gastric cancer and gastric inflammation. H. pylori induced upregulation in expression of integrin in gastric epithelia cells. Reactive oxygen species (ROS) are considered as an important regulator in the pathogenesis of H. pylori-induced gastric ulceration and carcinogenesis. Integrin expression may be regulated by oxidant-sensitive transcription factors, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori in aKorean isolate (HP99) induces the expression of integrin α5 and integrin β1, and whether H. pylori-induced expression of integrin α5 and integrin β1 are inhibited in the cells transfected with mutant genes for Ras (ras N-17), c-Jun (TAM-67), and IκBα(MAD-3) or treated with DPI, an inhibitor of NADPH oxidase. As a result, H. pylori induced the expression of integrin α5 and integrin β1 in gastric adenocarcinoma (AGS) cells time-dependently. Treatment of DPI or transfection with mutant genes for Ras (ras N-17), c-jun (TAM67), and IκBα(MAD3) inhibited H. pylori-induced expression of integrin α5 and integrin β1 in AGS cells. In conclusion, H. pylori activates Ras, NF-κB, and AP-1 and thus induces the expression of integrin α5 and integrin β1 in gastric epithelial cells. Inhibition of ROS production by DPI suppressed the expression of integrin α5 and integrin β1 in gastric epithelial cells. The results suggest the possible involvement of NADPH oxidase for ROS production in H. pylori-infected gastric epithelial cells.

UR - http://www.scopus.com/inward/record.url?scp=34247608221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247608221&partnerID=8YFLogxK

U2 - 10.1196/annals.1378.032

DO - 10.1196/annals.1378.032

M3 - Conference contribution

C2 - 17384273

AN - SCOPUS:34247608221

SN - 1573316458

SN - 9781573316453

T3 - Annals of the New York Academy of Sciences

SP - 298

EP - 304

BT - Signal Transduction Pathways, Part A

PB - Blackwell Publishing Inc.

ER -

Soon OC, Kyung HK, Yoon JH, Kim HY. Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells. In Signal Transduction Pathways, Part A: Apoptotic and Extracellular Signalling. Blackwell Publishing Inc. 2006. p. 298-304. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1378.032