Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells

Ok Cho Soon, Hwan Kim Kyung, Joo Heon Yoon, Hyeyoung Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

17 Citations (Scopus)

Abstract

Integrin expression in cancer tissues demonstrates its possible contribution to tumor progression, invasion, and metastasis. Helicobacter pylori (H. pylori) infection is related to gastric cancer and gastric inflammation. H. pylori induced upregulation in expression of integrin in gastric epithelia cells. Reactive oxygen species (ROS) are considered as an important regulator in the pathogenesis of H. pylori-induced gastric ulceration and carcinogenesis. Integrin expression may be regulated by oxidant-sensitive transcription factors, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). The present study aims to investigate whether H. pylori in aKorean isolate (HP99) induces the expression of integrin α5 and integrin β1, and whether H. pylori-induced expression of integrin α5 and integrin β1 are inhibited in the cells transfected with mutant genes for Ras (ras N-17), c-Jun (TAM-67), and IκBα(MAD-3) or treated with DPI, an inhibitor of NADPH oxidase. As a result, H. pylori induced the expression of integrin α5 and integrin β1 in gastric adenocarcinoma (AGS) cells time-dependently. Treatment of DPI or transfection with mutant genes for Ras (ras N-17), c-jun (TAM67), and IκBα(MAD3) inhibited H. pylori-induced expression of integrin α5 and integrin β1 in AGS cells. In conclusion, H. pylori activates Ras, NF-κB, and AP-1 and thus induces the expression of integrin α5 and integrin β1 in gastric epithelial cells. Inhibition of ROS production by DPI suppressed the expression of integrin α5 and integrin β1 in gastric epithelial cells. The results suggest the possible involvement of NADPH oxidase for ROS production in H. pylori-infected gastric epithelial cells.

Original languageEnglish
Title of host publicationSignal Transduction Pathways, Part A
Subtitle of host publicationApoptotic and Extracellular Signalling
PublisherBlackwell Publishing Inc.
Pages298-304
Number of pages7
ISBN (Print)1573316458, 9781573316453
DOIs
Publication statusPublished - 2006 Dec

Publication series

NameAnnals of the New York Academy of Sciences
Volume1090
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Soon, O. C., Kyung, H. K., Yoon, J. H., & Kim, H. (2006). Signaling for integrin α5/β1 expression in Helicobacter pylori-infected gastric epithelial AGS cells. In Signal Transduction Pathways, Part A: Apoptotic and Extracellular Signalling (pp. 298-304). (Annals of the New York Academy of Sciences; Vol. 1090). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1378.032