Signaling pathways for TNF production induced by human aminoacyl-tRNA synthetase-associating factor, p43

Heonyong Park, Sang Gyu Park, Junghee Kim, Young Gyu Ko, Sunghoon Kim

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51 Citations (Scopus)

Abstract

The p43 protein is associated with human macromolecular aminoacyl tRNA synthetase complex and secreted to up-regulate diverse proinflammatory genes including TNF. Here we focused on the p43-induced TNF production and determined its responsible signal pathway. The p43-induced TNF production was mediated by the activation of MAPK family members, ERK and p38 MAPK, and by IκB degradation leading to the activation of NFκB. We also studied the upstream molecules for ERK and p38 MAPK by using a variety of inhibitors. The inhibitors for protein kinase C (PKC) and phospholipase C (PLC) prevented the p43-induced TNF production. Interestingly, all of the effective drugs inhibited the ERK activity, while the drugs had no effects on p38 MAPK activity and IκB degradation. Together, the p43-induced TNF production was controlled by NFkB, p38 MAPK, and ERK that is dependent on the activities of PLC and PKC.

Original languageEnglish
Pages (from-to)148-153
Number of pages6
JournalCytokine
Volume20
Issue number4
DOIs
Publication statusPublished - 2002 Nov

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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