SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy

Ryuichi Katayama, Yuzuru Takashima, No Cheol Park, Geon Lim, Won Sup Lee, Hyungbae Moon, Guk Jong Choi, Young Pil Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have characterized a new type STED microscope which combines a high numerical aperture (NA) optical head with a solid immersion lens (SIL), and we call it as SIL-STED microscope. The advantage of a SIL-STED microscope is that its high NA of the SIL makes it superior to a general STED microscope in lateral resolution, thus overcoming the optical diffraction limit at the macromolecular level and enabling advanced super-resolution imaging of cell surface or cell membrane structure and function Do. This study presents the first implementation of higher NA illumination in a STED microscope limiting the fluorescence lateral resolution to about 40 nm. The refractive index of the SIL which is made of material KTaO3 is about 2.23 and 2.20 at a wavelength of 633 nm and 780 nm which are used for excitation and depletion in STED imaging, respectively. Based on the vector diffraction theory, the electric field focused by the SILSTED microscope is numerically calculated so that the numerical results of the point dispersion function of the microscope and the expected resolution could be analyzed. For further investigation, fluorescence imaging of nano size fluorescent beads is fulfilled to show improved performance of the technique.

Original languageEnglish
Title of host publicationOptical Data Storage 2017
Subtitle of host publicationFrom New Materials to New Systems
PublisherSPIE
Volume10384
ISBN (Electronic)9781510612259
DOIs
Publication statusPublished - 2017 Jan 1
EventOptical Data Storage 2017: From New Materials to New Systems, ODS 2017 - San Diego, United States
Duration: 2017 Aug 6 → …

Other

OtherOptical Data Storage 2017: From New Materials to New Systems, ODS 2017
CountryUnited States
CitySan Diego
Period17/8/6 → …

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Fluorescence Microscopy
Super-resolution
Fluorescence microscopy
Immersion
Microscopy
Microscope
submerging
Lens
Lenses
Microscopic examination
Microscopes
microscopes
lenses
microscopy
fluorescence
numerical aperture
Imaging
Imaging techniques
Fluorescence
Lateral

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Katayama, R., Takashima, Y., Park, N. C., Lim, G., Lee, W. S., Moon, H., ... Park, Y. P. (2017). SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy. In Optical Data Storage 2017: From New Materials to New Systems (Vol. 10384). [103840F] SPIE. https://doi.org/10.1117/12.2275957
Katayama, Ryuichi ; Takashima, Yuzuru ; Park, No Cheol ; Lim, Geon ; Lee, Won Sup ; Moon, Hyungbae ; Choi, Guk Jong ; Park, Young Pil. / SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy. Optical Data Storage 2017: From New Materials to New Systems. Vol. 10384 SPIE, 2017.
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Katayama, R, Takashima, Y, Park, NC, Lim, G, Lee, WS, Moon, H, Choi, GJ & Park, YP 2017, SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy. in Optical Data Storage 2017: From New Materials to New Systems. vol. 10384, 103840F, SPIE, Optical Data Storage 2017: From New Materials to New Systems, ODS 2017, San Diego, United States, 17/8/6. https://doi.org/10.1117/12.2275957

SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy. / Katayama, Ryuichi; Takashima, Yuzuru; Park, No Cheol; Lim, Geon; Lee, Won Sup; Moon, Hyungbae; Choi, Guk Jong; Park, Young Pil.

Optical Data Storage 2017: From New Materials to New Systems. Vol. 10384 SPIE, 2017. 103840F.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Katayama R, Takashima Y, Park NC, Lim G, Lee WS, Moon H et al. SIL-STED microscopy technique enhancing super-resolution of fluorescence microscopy. In Optical Data Storage 2017: From New Materials to New Systems. Vol. 10384. SPIE. 2017. 103840F https://doi.org/10.1117/12.2275957