Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants

Kyoung Sil Yang, Hee Seung Kim, Un Ho Jin, Sang Sook Lee, Jong A. Park, Yong Pyo Lim, Hyun Sook Pai

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

BTF3 (βNAC) was originally isolated as a general transcription factor required for RNA polymerase II-dependent transcription, and later found to be a β-subunit of nascent-polypeptide-associated complex that has been implicated in regulating protein localization during translation. In this study, virus-induced gene silencing of NbBTF3 encoding a Nicotiana benthamiana homolog of human BTF3 caused leaf yellowing and abnormal leaf morphology without altering the overall growth of the plant. The NbBTF3 gene is constitutively expressed and the NbBTF3-GFP fusion protein is primarily targeted to the nucleus. At the cellular level, downregulation of NbBTF3 expression reduced the chloroplast sizes and chlorophyll contents. The affected cells produced excessive amounts of reactive oxygen species. Furthermore, the transcript level of various plastid- and mitochondria-encoded genes was severely reduced in the NbBTF3-depleted leaf cells. These findings indicate that depletion of NbBTF3 activity preferentially affected development and/or physiology of chloroplasts and mitochondria in plants, possibly by hampering efficient translocation of the nascent organellar proteins into the organelles.

Original languageEnglish
Pages (from-to)1459-1469
Number of pages11
JournalPlanta
Volume225
Issue number6
DOIs
Publication statusPublished - 2007 May 1

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Chloroplasts
Mitochondria
mitochondria
chloroplasts
Gene Expression
gene expression
General Transcription Factors
leaves
Proteins
Plastids
proteins
Nicotiana benthamiana
RNA Polymerase II
Gene Silencing
gene silencing
Chlorophyll
DNA-directed RNA polymerase
Organelles
translation (genetics)
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Yang, Kyoung Sil ; Kim, Hee Seung ; Jin, Un Ho ; Lee, Sang Sook ; Park, Jong A. ; Lim, Yong Pyo ; Pai, Hyun Sook. / Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants. In: Planta. 2007 ; Vol. 225, No. 6. pp. 1459-1469.
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Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants. / Yang, Kyoung Sil; Kim, Hee Seung; Jin, Un Ho; Lee, Sang Sook; Park, Jong A.; Lim, Yong Pyo; Pai, Hyun Sook.

In: Planta, Vol. 225, No. 6, 01.05.2007, p. 1459-1469.

Research output: Contribution to journalArticle

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